CAJ | 학술논문

从木材识别的角度，对木材DNA提取方法（ CTAB、SDS、PTB、DNeasy Plant Mini Kit等）和DNA条形码及DNA指纹图谱等基于DNA的木材树种识别方法进行了综合评述。其中，针对DNA条形码方法，重点阐述了基因组DNA中可用的特征序列来源叶绿体基因（ chloroplast DNA， cpDNA） rbcL、matK、trnH-psbA间隔区序列，核糖体基因（ ribosomal DNA， rDNA） ITS序列，以及变异位点的识别和系统进化树的应用等问题；针对DNA指纹图谱方法，重点阐述了（RAPD、ISSR、SSR、SNP）4种DNA分子标记方法在DNA指纹图谱中的研究和应用现状。笔者认为，以DNA特征序列为依据的木材树种识别理论上虽然是可行的，但要应用于实际还需开展更多的研究。
종목재식별적각도，대목재DNA제취방법（ CTAB、SDS、PTB、DNeasy Plant Mini Kit등）화DNA조형마급DNA지문도보등기우DNA적목재수충식별방법진행료종합평술。기중，침대DNA조형마방법，중점천술료기인조DNA중가용적특정서렬래원협록체기인（ chloroplast DNA， cpDNA） rbcL、matK、trnH-psbA간격구서렬，핵당체기인（ ribosomal DNA， rDNA） ITS서렬，이급변이위점적식별화계통진화수적응용등문제；침대DNA지문도보방법，중점천술료（RAPD、ISSR、SSR、SNP）4충DNA분자표기방법재DNA지문도보중적연구화응용현상。필자인위，이DNA특정서렬위의거적목재수충식별이론상수연시가행적，단요응용우실제환수개전경다적연구。
From wood identification points of view, DNA extraction methods ( CTAB, SDS, PTB, DNeasy Plant Mini Kit etc.) and DNA barcode and fingerprint and wood identification methods based on DNA were summarized. For the DNA barcode concerned, the emphases were on the signature sequences in genome DNA ( chloroplast DNA, cpDNAr-bcL,matK,trnH-psbAintergenic spacer sequences,and ribosomal DNA, rDNA ITS) as well as identification form ( varia-ble sites and phylogenetic tree) described. For the DNA fingerprint method,the emphases were on the progress and out-look of 4 kinds of molecular makers such as random amplificated polymorphism DNA, RAPD,inter-simple sequence re-peat, ISSR、simple sequence repeats, SSR,single nucleotide polymorphism, SNP. It was concluded that wood identifica-tion based on DNA methods is feasible in theory but needs more studied on.