CAJ | 학술논문

橡胶树死皮是影响天然橡胶产量的重要因子之一。采用双向凝胶电泳对健康树和死皮树橡胶粒子中差异表达的蛋白进行分离，分析获得35个差异表达的蛋白点，通过基质辅助激光解析电离飞行时间质谱（ MALDI-TOF MS）分析及搜索Uniprot rubber数据库后，有13个蛋白点被成功鉴定，其中橡胶延长因子（ rubber elongation factor，REF ）、法尼基焦磷酸合成酶（ Ffarnesyl-diphosphate synthase， FPS ）、谷胱甘肽过氧化酶（ glutathione peroxidase，GPX）、谷胱甘肽还原酶（glutathione reductase，GR）、翻译控制肿瘤蛋白（translationally controlled tumor protein，TCTP）、热激蛋白（heat shock protein， HSP）等主要蛋白参与了橡胶的生物合成、活性氧代谢及细胞凋亡过程。说明橡胶的生物合成、活性氧代谢及细胞凋亡途径可能是橡胶树死皮发生的关键调控途径。
상효수사피시영향천연상효산량적중요인자지일。채용쌍향응효전영대건강수화사피수상효입자중차이표체적단백진행분리，분석획득35개차이표체적단백점，통과기질보조격광해석전리비행시간질보（ MALDI-TOF MS）분석급수색Uniprot rubber수거고후，유13개단백점피성공감정，기중상효연장인자（ rubber elongation factor，REF ）、법니기초린산합성매（ Ffarnesyl-diphosphate synthase， FPS ）、곡광감태과양화매（ glutathione peroxidase，GPX）、곡광감태환원매（glutathione reductase，GR）、번역공제종류단백（translationally controlled tumor protein，TCTP）、열격단백（heat shock protein， HSP）등주요단백삼여료상효적생물합성、활성양대사급세포조망과정。설명상효적생물합성、활성양대사급세포조망도경가능시상효수사피발생적관건조공도경。
The tapping panel dryness ( TPD) in Hevea brasiliensis is one of the important factors influencing natural rub-ber production. In order to distinguish and identify the differentially expressed proteins of latex rubber particles in healthy and TPD trees, the two-dimensional electrophoresis (2-DE) was used. 35 differentially expressed protein spots were ob-tained by software analysis. Subjecting to MALDI-TOF MS following by searching for Uniprot rubber database, 13 protein spots were successfully identified, Of them, REF, FPS, GPX, GR, TCTP and HSP were mainly involed in the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis processes. This study further showed that the pathways of the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis were possibly the key regulated ones of TPD and which would provide a theoretical basis for elucidating the TPD mechanism in Hevea brasiliensis.