CAJ | 학술논문

目的：探讨贵州从江侗族人群外周血白细胞趋化因子受体CCR5（CCR5）基因的mRNA表达与急性乙型肝炎（AHB组）、慢性乙型肝炎（CHB组）的相关性。方法选取侗族人群AHB感染者20例（AHB组）、CHB患者32例（CHB组），以同期该区健康侗族人群50例作为对照组，Trizol-酚-氯仿一步法提取外周血白细胞总 RNA，应用实时荧光定量 RQ-PCR技术检测外周血CCR5 mRNA表达水平。结果 AHB组外周血CCR5 mRNA相对表达量为（1．1199±0．7233），CHB组为（0．5823±0．2736），对照组外周血CCR5 mRNA相对表达量为（0．7985±0．3492）。AHB组CCR5 mRNA表达水平显著高于对照组，CHB组CCR5 mRNA表达水平显著低于对照组，AHB组CCR5 mRNA表达水平显著高于 CHB组，差异均有统计学意义（P＜0．05）；而CCR5 mRNA相对表达水平与患者性别、年龄无相关性（P＞0．05）。结论 CCR5可能与 AHB、CHB具有相关性，CCR5 mRNA的表达强度很可能与机体清除乙型肝炎病毒（HBV）的能力呈正相关，高水平的CCR5 mRNA可能不利于 HBV的复制。
목적：탐토귀주종강동족인군외주혈백세포추화인자수체CCR5（CCR5）기인적mRNA표체여급성을형간염（AHB조）、만성을형간염（CHB조）적상관성。방법선취동족인군AHB감염자20례（AHB조）、CHB환자32례（CHB조），이동기해구건강동족인군50례작위대조조，Trizol-분-록방일보법제취외주혈백세포총 RNA，응용실시형광정량 RQ-PCR기술검측외주혈CCR5 mRNA표체수평。결과 AHB조외주혈CCR5 mRNA상대표체량위（1．1199±0．7233），CHB조위（0．5823±0．2736），대조조외주혈CCR5 mRNA상대표체량위（0．7985±0．3492）。AHB조CCR5 mRNA표체수평현저고우대조조，CHB조CCR5 mRNA표체수평현저저우대조조，AHB조CCR5 mRNA표체수평현저고우 CHB조，차이균유통계학의의（P＜0．05）；이CCR5 mRNA상대표체수평여환자성별、년령무상관성（P＞0．05）。결론 CCR5가능여 AHB、CHB구유상관성，CCR5 mRNA적표체강도흔가능여궤체청제을형간염병독（HBV）적능력정정상관，고수평적CCR5 mRNA가능불리우 HBV적복제。
Objective To investigate the expression of CCR5 gene mRNA in peripheral blood of the population between Con-gj iang Dong minority from Guizhou,and to evaluate the relevance between CCR5 gene and hepatitis B infection.Methods A total of twenty individuals with AHB infection(AHB group,20 cases),thirty individuals with CHB infection(CHB group,32 cases),Fifty healthy controls of peripheral blood were recruited to conduct a case-control study among Dong ethnicity.Total RNA from blood was extracted and purified by the trizol-phenol-chloroform one-step method.Expression of CCR5 mRNA was detected by using SYBR green supermix real-time fluorescent quantitative PCR.Results The relative expression of CCR5 mRNA in AHB infectiong-roup was (1.119 9±0.723 3),the data of CCR5 mRNA in CHB group was (0.582 3±0.273 6),the data of CCR5 mRNA in con-trol group was (0.798 5±0.349 2)respectively.The results showed that the expression level of CCR5 mRNA in AHB group was significantly higher than that of the control group,CCR5 mRNA expression was lower in CHB group than in control group,the ex-pression level of CCR5 mRNA in AHB group was significantly higher than CHB group(P<0.05),However the relative expression of CCR5 mRNA had no correlation with age and gender(P>0.05).Conclusion CCR5 gene is associated with AHB and CHB,there is positive correlation between the expression instensity of CCR5 mRNA and the clearance degrees of HBV possibly.