CAJ | 학술논문

建立离子色谱测定果汁中的乳酸、富马酸、柠檬酸的分析方法。利用Onguard II RP柱进行预处理除去疏水性物质，使用IonPac AS19(250 mm×4 mm)色谱柱，IonPac AG19(250 mm×4 mm)保护柱，以氢氧化钾溶液梯度淋洗，采用自循环抑制电导检测的方法对苹果汁样品中的乳酸、富马酸、柠檬酸含量进行检测。该方法能够准确地测定果汁中乳酸、富马酸、柠檬酸的含量，线性方程的相关系数在0.999以上，样品的加标回收率为90.7%～102.8%，测定结果的相对标准偏差为0.41%～1.43%(n=6)，样品的定量限分别为0.5，0.2，0.3 mg/L。该法操作简单，环境友好，灵敏度高，测定结果准确可靠，能满足果汁中有机酸含量的测定要求。
건립리자색보측정과즙중적유산、부마산、저몽산적분석방법。이용Onguard II RP주진행예처리제거소수성물질，사용IonPac AS19(250 mm×4 mm)색보주，IonPac AG19(250 mm×4 mm)보호주，이경양화갑용액제도림세，채용자순배억제전도검측적방법대평과즙양품중적유산、부마산、저몽산함량진행검측。해방법능구준학지측정과즙중유산、부마산、저몽산적함량，선성방정적상관계수재0.999이상，양품적가표회수솔위90.7%～102.8%，측정결과적상대표준편차위0.41%～1.43%(n=6)，양품적정량한분별위0.5，0.2，0.3 mg/L。해법조작간단，배경우호，령민도고，측정결과준학가고，능만족과즙중유궤산함량적측정요구。
A new method for determination of lactate,fumarate and citrate in fruit juice was developed and validated based on ion chromatography. Onguard II RP cartridges were used to remove hydrophobic substances in the fruit juice. The content of lactate,fumarate and citrate in fruit juice were determined by using IonPac AS19 chromatographic column and IonPac AG19 guard column, potassium hydroxide gradient elution and circulation suppression conductometric. The calibration curves of correlation coefficients were more than 0.999 for these organic acids. The recoveries were between 90.7%-102.8%and the relative standard deviation of determination results was between 0.41%-1.43%(n=6). The detection limits were 0.5,0.2,0.3 mg/L for lactate,fumarate and citrate, respectively. The method is environmental friendly and has high sensitivity,good selectivity, and can meet the requirement of the determination of organic acids content in fruit juice.