CAJ | 학술논문

背景与目的：大量研究表明，Ezrin蛋白在很多肿瘤组织中都有异常表达，参与细胞的多种生物学行为，如细胞黏附、迁移、生存等，在肿瘤的发生、发展过程中扮演重要的角色。本研究旨在分析Ezrin蛋白在正常宫颈组织和宫颈癌组织中的表达差异及与临床病理因素、PCNA、MMP-2、MMP-9、HPV E6蛋白之间的相互关系。方法：收集2002年1月-2006年12月在复旦大学附属妇产科医院妇科诊断为宫颈癌的30例患者的组织标本，并进行回顾性研究。同时收集正常宫颈组织来自子宫肌瘤且经病理证实无任何宫颈病变的10例组织标本作为对照。应用免疫组织化学方法检测宫颈癌组织和正常宫颈组织中Ezrin、PCNA、MMP-2、MMP-9、HPV E6蛋白表达，并分析Ezrin表达与PCNA、MMP-2、MMP-9、HPV E6蛋白表达的关系。结果：Ezrin蛋白在宫颈癌组织中表达呈中强阳性，且主要弥散分布在细胞质和细胞膜处，而正常宫颈组织Ezrin蛋白表达呈弱阳性，且主要分布在细胞膜处，差异有统计学意义(P<0.05)。Ezrin蛋白在宫颈组织中的表达与MMP-2、MMP-9和HPV E6蛋白在宫颈组织中的表达呈正相关，与PCNA表达无关。Ezrin蛋白表达与年龄、肿瘤大小、病理类型、组织分化程度、临床分期等关系差异无统计学意义(P>0.05)。结论：Ezrin在宫颈癌组织中高表达，可能参与宫颈癌侵袭转移。
배경여목적：대량연구표명，Ezrin단백재흔다종류조직중도유이상표체，삼여세포적다충생물학행위，여세포점부、천이、생존등，재종류적발생、발전과정중분연중요적각색。본연구지재분석Ezrin단백재정상궁경조직화궁경암조직중적표체차이급여림상병리인소、PCNA、MMP-2、MMP-9、HPV E6단백지간적상호관계。방법：수집2002년1월-2006년12월재복단대학부속부산과의원부과진단위궁경암적30례환자적조직표본，병진행회고성연구。동시수집정상궁경조직래자자궁기류차경병리증실무임하궁경병변적10례조직표본작위대조。응용면역조직화학방법검측궁경암조직화정상궁경조직중Ezrin、PCNA、MMP-2、MMP-9、HPV E6단백표체，병분석Ezrin표체여PCNA、MMP-2、MMP-9、HPV E6단백표체적관계。결과：Ezrin단백재궁경암조직중표체정중강양성，차주요미산분포재세포질화세포막처，이정상궁경조직Ezrin단백표체정약양성，차주요분포재세포막처，차이유통계학의의(P<0.05)。Ezrin단백재궁경조직중적표체여MMP-2、MMP-9화HPV E6단백재궁경조직중적표체정정상관，여PCNA표체무관。Ezrin단백표체여년령、종류대소、병리류형、조직분화정도、림상분기등관계차이무통계학의의(P>0.05)。결론：Ezrin재궁경암조직중고표체，가능삼여궁경암침습전이。
Background and purpose:Previous studies have revealed potential roles for Ezrin in a variety of cancers. Ezrin participates in several functions including cell adhesion, motility and cell survival, and there is increasing evidence that it regulates tumour progression. This study aimed to determine the differential expression of Ezrin between cervical cancer tissues and normal controls and to explore the association of Ezrin expression with clinical pathological characteristics and with the expression of PCNA, MMP-2, MMP-9 and HPV E6 protein. Methods:Retrospective study was performed among 30 cervical cancer tissue samples which were diagnosed in our hospital from Jan. 2002 to Dec. 2006. Ten normal cervixes from uterine myomas were employed as normal controls. Semi-quantitative immunohistochemisty analysis was performed to detect the association of Ezrin expression with clinical pathological characteristics, and with expression of PCNA, MMP-2 and MMP-9, HPV E6 protein in cervical cancer tissue and normal controls. Analyze the association of Ezrin expression with the clinical characteristics. Results:The expression of Ezrin was strong and the distribution was diffuse mainly in the cytoplasm and membrane in cervical cancer tissues, while the normal cervical tissues was weak and mainly in the membrane. However, there was no signiifcant association between the expression of Ezrin and the clinical characteristics including tumor stage, histology and grade. Ezrin expression was positively correlated with the expression of MMP-2, MMP-9 and HPV E6 protein in cervical cancer tissues, except PCNA. Conclusion:Ezrin was over-expressed in cervical cancer tissues and may be involved in the progress of invasion and metastasis in cervical cancer.