CAJ | 학술논문

卵黄蛋白原（Vitellogenin，Vtg）是检测环境雌激素的重要生物标志物，雄性鱼类Vtg mRNA是检测环境雌激素的新兴生物标志物。为研究BaP是否具有环境雌激素效应，本试验对罗非鱼Oreochromis niloticus Vtg mRNA进行研究，用总RNA提取试剂盒提取得到雌鱼肝脏总RNA，以转录得到的cDNA为模板，以Primer设计得到的引物，得到422 bp的罗非鱼卵黄蛋白原特异性cDNA扩增片段；采用荧光定量PCR法检测10、50μg·L-1的BaP暴露对罗非鱼肝脏Vtg基因表达的影响，结果发现4 d、17 d时雄性罗非鱼肝脏内Vtg mRNA表达量与对照组无差异（P>0.05），10 d时10、50μg·L-12个剂量组Vtg mRNA表达量显著高于对照组（P<0.01），分别是对照组的113.18倍和121.79倍。结果意味着BaP可能具有环境雌激素效应。
란황단백원（Vitellogenin，Vtg）시검측배경자격소적중요생물표지물，웅성어류Vtg mRNA시검측배경자격소적신흥생물표지물。위연구BaP시부구유배경자격소효응，본시험대라비어Oreochromis niloticus Vtg mRNA진행연구，용총RNA제취시제합제취득도자어간장총RNA，이전록득도적cDNA위모판，이Primer설계득도적인물，득도422 bp적라비어란황단백원특이성cDNA확증편단；채용형광정량PCR법검측10、50μg·L-1적BaP폭로대라비어간장Vtg기인표체적영향，결과발현4 d、17 d시웅성라비어간장내Vtg mRNA표체량여대조조무차이（P>0.05），10 d시10、50μg·L-12개제량조Vtg mRNA표체량현저고우대조조（P<0.01），분별시대조조적113.18배화121.79배。결과의미착BaP가능구유배경자격소효응。
Vitellogenin (Vtg) is an important biomarker for monitoring environmental estrogens (EEs) in aquatic environment. Vtg mRNA in male fish is a new kind of biomarker. In order to research whether BaP has environmental estrogen effect, the Vtg mRNA was also studied in this paper. Total liver RNA was extracted from the liver by Trizol kit. The RT-PCR was conducted with vitellogenin special primers designed by Esterhuyse and by ourselves. The isolated 422 bp tilapia vitellogenin cDNA were obtained. The effect of BaP on tilapia liver Vtg mRNA expression was detected with the help of real-time PCR. Results showed that there were no significant differences of Vtg gene expression among control group and the exposure concentrations of 10μg·L-1 and 50μg·L-1 in day 4 and 17(P>0.05), in day 10, the Vtg mRNA level increased by 113.18%and 121.79%respectively in 10μg·L-1 group and 50μg·L-1 group which were significantly higher than that in control group (P<0.01).