农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2013年
11期
1575-1577,1582
,共4页
樟子松%松针%石蜡切片
樟子鬆%鬆針%石蠟切片
장자송%송침%석사절편
Pinus sylvestris%Pine needles%Paraffin section
[目的]以樟子松松针为材料，探索一套适合樟子松松针制备石蜡切片的条件。[方法]在传统植物石蜡切片技术的基础上，结合松针的结构特点，对固定、脱水、粘片、染色等环节进行了系列摸索。[结果]FAA固定剂配比中采用70%乙醇；粘片时材料置于40℃水浴锅中展开，用防脱载玻片捞取，置35℃烘箱24 h；松针切8μm厚度,染色条件为伊红5 min，苏木精1 min。[结论]获得的条件可强化固定和脱水效果，粘片操作简便，不易脱片，染色效果较好。
[目的]以樟子鬆鬆針為材料，探索一套適閤樟子鬆鬆針製備石蠟切片的條件。[方法]在傳統植物石蠟切片技術的基礎上，結閤鬆針的結構特點，對固定、脫水、粘片、染色等環節進行瞭繫列摸索。[結果]FAA固定劑配比中採用70%乙醇；粘片時材料置于40℃水浴鍋中展開，用防脫載玻片撈取，置35℃烘箱24 h；鬆針切8μm厚度,染色條件為伊紅5 min，囌木精1 min。[結論]穫得的條件可彊化固定和脫水效果，粘片操作簡便，不易脫片，染色效果較好。
[목적]이장자송송침위재료，탐색일투괄합장자송송침제비석사절편적조건。[방법]재전통식물석사절편기술적기출상，결합송침적결구특점，대고정、탈수、점편、염색등배절진행료계렬모색。[결과]FAA고정제배비중채용70%을순；점편시재료치우40℃수욕과중전개，용방탈재파편로취，치35℃홍상24 h；송침절8μm후도,염색조건위이홍5 min，소목정1 min。[결론]획득적조건가강화고정화탈수효과，점편조작간편，불역탈편，염색효과교호。
[Objective] Taking Pinus sylvestris needles as materials, a set of test con-ditions suitable for the preparation of paraffin sections of Pinus sylvestris needles was determined. [Method] Based on the traditional method for paraffin sections preparation, steps including fixation, dehydration, adhering to slides and staining were investigated taking the structural characteristics of pine needles into considera-tion. [Result] 70% ethanol was used in the FAA fixative; before affixed on slides, the cut sections were first expanded in a 40 ℃ water bath and taken out by using Su-perfrost Plus slides, fol owed by drying in a 35 ℃ oven for 24 h; pine needles were cut into 8 μm thick sections; staining was achieved by immersion in eosin for 5 min and in hematoxylin for 1 min. [Conclusion] The conditions obtained above can en-hance the effect of fixation and dehydration; adhering to slides is easy to operate and the sections are not easy to drop; the dyeing effect is relatively preferable.
