CAJ | 학술논문

目的：制备脑心肌炎病毒（ EMCV）实验性疫苗并对其免疫原性进行初步研究。方法采用Vero细胞培养EMCV病毒，微量细胞病变法检测病毒滴度；经过浓缩、过滤、超速离心等方法初步纯化EMCV；摸索病毒灭活方法；确定病毒半数致死量（ LD50）；通过肌肉注射途径免疫小鼠，微量中和试验测定血清中和抗体效价；免疫后小鼠分别以100LD50、50LD50和25LD50进行腹腔攻毒试验。结果以1∶4000β-丙内酯4℃放置12 h作为病毒灭活条件，病毒半数致死量17 CCID50/ml；免疫小鼠能检测到中和抗体并对病毒攻击具有抵抗力。结论 EMCV实验性疫苗能诱导小鼠产生保护性抗体。
목적：제비뇌심기염병독（ EMCV）실험성역묘병대기면역원성진행초보연구。방법채용Vero세포배양EMCV병독，미량세포병변법검측병독적도；경과농축、과려、초속리심등방법초보순화EMCV；모색병독멸활방법；학정병독반수치사량（ LD50）；통과기육주사도경면역소서，미량중화시험측정혈청중화항체효개；면역후소서분별이100LD50、50LD50화25LD50진행복강공독시험。결과이1∶4000β-병내지4℃방치12 h작위병독멸활조건，병독반수치사량17 CCID50/ml；면역소서능검측도중화항체병대병독공격구유저항력。결론 EMCV실험성역묘능유도소서산생보호성항체。
Objective To prepare an experimental encephalomyocarditis virus ( EMCV) vaccine and study its immunogenicity .Methods EMCVs were cultured in Vero cells and viral titer was detected by micro cytopathy method .A series of procedures including concentration , filtration and ultracentrifugation were conducted to purify EMCVs .Formaldehyde and β-propiolactone were used for viral inactivation and their efficacy was evaluated .The median lethal dose ( LD50 ) of EMCV was measured by using Reed-Muench method.The serum antibody titers in BALB/c mice vaccinated with different immunization strategies were analyzed through microneutralization assay .Then the immunized mice were challenged with 100LD50 , 50LD50 and 25LD50 of EMCV by intraperitoneal injection .Results The viruses could be inactivated by β-propiolactone at a volume ratio of 1 ∶4000 for 12 hours at 4℃.The LD50 of EMCV was 17 CCID50/ml.The immunized mice produced neutralizing antibodies and displayed resistance to EMCV infection.Conclusion The experimental EMCV vaccine could induce protective antibodies in mice .