现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
8期
271-272
,共2页
高效液相色谱(HPLC)%方便榨菜%安赛蜜%苯甲酸%山梨酸%糖精钠%脱氢乙酸
高效液相色譜(HPLC)%方便榨菜%安賽蜜%苯甲痠%山梨痠%糖精鈉%脫氫乙痠
고효액상색보(HPLC)%방편자채%안새밀%분갑산%산리산%당정납%탈경을산
high performance liquid chromatography ( HPLC )%convenience pickled mustard tubers%acesulfame%benzoic acid%sorbic acid%sodium saccharin%dehydroacetate
建立高效液相色谱法同时测定方便榨菜中安赛蜜、苯甲酸、山梨酸、糖精钠及脱氢乙酸5种食品添加剂的检测方法。采用高效液相色谱法,Waters SymmetryShield C18(150 mm×4.6 mm,5μm)色谱柱进行分离,流动相为0.02 mol/L乙酸铵缓冲溶液∶甲醇(95∶5,V/V),流速1.0 mL/min,检测波长220 nm,一次直接进样,同时测定5种食品添加剂。各物质在0.50~100.0 mg/kg范围内呈良好的线性关系,相关系数为0.9990~0.9999,精密度良好,加标回收率92.5%~98.8%,RSD<3%。该方法准确度较高,重现性好,操作简便,能在20 min内将5种添加剂完全分离,适用于大批量方便榨菜中常用食品添加剂的日常检测。
建立高效液相色譜法同時測定方便榨菜中安賽蜜、苯甲痠、山梨痠、糖精鈉及脫氫乙痠5種食品添加劑的檢測方法。採用高效液相色譜法,Waters SymmetryShield C18(150 mm×4.6 mm,5μm)色譜柱進行分離,流動相為0.02 mol/L乙痠銨緩遲溶液∶甲醇(95∶5,V/V),流速1.0 mL/min,檢測波長220 nm,一次直接進樣,同時測定5種食品添加劑。各物質在0.50~100.0 mg/kg範圍內呈良好的線性關繫,相關繫數為0.9990~0.9999,精密度良好,加標迴收率92.5%~98.8%,RSD<3%。該方法準確度較高,重現性好,操作簡便,能在20 min內將5種添加劑完全分離,適用于大批量方便榨菜中常用食品添加劑的日常檢測。
건립고효액상색보법동시측정방편자채중안새밀、분갑산、산리산、당정납급탈경을산5충식품첨가제적검측방법。채용고효액상색보법,Waters SymmetryShield C18(150 mm×4.6 mm,5μm)색보주진행분리,류동상위0.02 mol/L을산안완충용액∶갑순(95∶5,V/V),류속1.0 mL/min,검측파장220 nm,일차직접진양,동시측정5충식품첨가제。각물질재0.50~100.0 mg/kg범위내정량호적선성관계,상관계수위0.9990~0.9999,정밀도량호,가표회수솔92.5%~98.8%,RSD<3%。해방법준학도교고,중현성호,조작간편,능재20 min내장5충첨가제완전분리,괄용우대비량방편자채중상용식품첨가제적일상검측。
Detection method for simultaneous determination of 5 kinds of food additives namely acesulfame ,benzoic acid,sorbic acid,sodium saccharin,dehydroacetate in convenience pickled mustard tubers by HPLC was made.Using high performance liquid chromatography ,Waters Symmetry Shield C18(150 mm×4.6 mm,5μm) were isolated from the column,the mobile phase was 0.02 mol/L ammonium acetate buffer solution∶methanol(95∶5, V/V),flow rate of 1.0 mL/min,the detection wave length was 220 nm,a direct injection,simultaneous determination of 5 food additives.The materials in the 0.50~100.0 mg/kg showed good linear relationships ,the correlation coefficient were 0.999 0~0.999 9 ,good precision ,recovery rate were 92.5%~98.8%,RSD<3%. The method had high accuracy ,and it had good reproducibility ,and simple operation ,can be completely separated within 20 min of 5 kinds of additives ,applicable for routine determination of common food additives in large quantities of convenience pickled mustard tubers.