中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
21期
3345-3349
,共5页
生物材料%纳米材料%聚乙二醇接枝支化聚乙烯亚胺%阳离子脂质体%基因转染%螺旋神经节细胞%绿色荧光蛋白%广东省自然科学基金
生物材料%納米材料%聚乙二醇接枝支化聚乙烯亞胺%暘離子脂質體%基因轉染%螺鏇神經節細胞%綠色熒光蛋白%廣東省自然科學基金
생물재료%납미재료%취을이순접지지화취을희아알%양리자지질체%기인전염%라선신경절세포%록색형광단백%광동성자연과학기금
polyethylene glycols%polyethyleneimine%liposomes%spiral ganglion
背景：新型阳离子纳米材料叶酸聚乙二醇接枝支化聚乙烯亚胺易于合成和改性，稳定性好，结构及性能容易调控，已被作为基因治疗转染载体应用于多种疾病的研究。<br> 目的：观察叶酸聚乙二醇接枝支化聚乙烯亚胺转染体外培养小鼠内耳螺旋神经节细胞的可行性及特点。<br> 方法：分别以叶酸聚乙二醇接枝支化聚乙烯亚胺(实验组)、阳离子脂质体Lipofectamine?2000(对照组)作为载体，制备携带增强型绿色荧光蛋白的转染载体系统。将昆明小鼠螺旋神经节细胞分别培养于含实验组和对照组转染载体系统的DMEM/F-12培养基中，培养24 h后，MTT法观察载体系统的细胞毒性，倒置荧光显微镜观察细胞形态及绿色荧光蛋白表达强度，流式细胞仪检测细胞转染率。<br> 结果与结论：两种载体分别转染后，螺旋神经节细胞形态均未发生明显改变，但两组载体对螺旋神经节细胞均有一定的毒性，实验组载体毒性较小且转染效率较高(P<0.05)。结果说明叶酸聚乙二醇接枝支化聚乙烯亚胺基因转染体外螺旋神经节细胞的转染效率及细胞毒性均优于传统的阳离子脂质体。
揹景：新型暘離子納米材料葉痠聚乙二醇接枝支化聚乙烯亞胺易于閤成和改性，穩定性好，結構及性能容易調控，已被作為基因治療轉染載體應用于多種疾病的研究。<br> 目的：觀察葉痠聚乙二醇接枝支化聚乙烯亞胺轉染體外培養小鼠內耳螺鏇神經節細胞的可行性及特點。<br> 方法：分彆以葉痠聚乙二醇接枝支化聚乙烯亞胺(實驗組)、暘離子脂質體Lipofectamine?2000(對照組)作為載體，製備攜帶增彊型綠色熒光蛋白的轉染載體繫統。將昆明小鼠螺鏇神經節細胞分彆培養于含實驗組和對照組轉染載體繫統的DMEM/F-12培養基中，培養24 h後，MTT法觀察載體繫統的細胞毒性，倒置熒光顯微鏡觀察細胞形態及綠色熒光蛋白錶達彊度，流式細胞儀檢測細胞轉染率。<br> 結果與結論：兩種載體分彆轉染後，螺鏇神經節細胞形態均未髮生明顯改變，但兩組載體對螺鏇神經節細胞均有一定的毒性，實驗組載體毒性較小且轉染效率較高(P<0.05)。結果說明葉痠聚乙二醇接枝支化聚乙烯亞胺基因轉染體外螺鏇神經節細胞的轉染效率及細胞毒性均優于傳統的暘離子脂質體。
배경：신형양리자납미재료협산취을이순접지지화취을희아알역우합성화개성，은정성호，결구급성능용역조공，이피작위기인치료전염재체응용우다충질병적연구。<br> 목적：관찰협산취을이순접지지화취을희아알전염체외배양소서내이라선신경절세포적가행성급특점。<br> 방법：분별이협산취을이순접지지화취을희아알(실험조)、양리자지질체Lipofectamine?2000(대조조)작위재체，제비휴대증강형록색형광단백적전염재체계통。장곤명소서라선신경절세포분별배양우함실험조화대조조전염재체계통적DMEM/F-12배양기중，배양24 h후，MTT법관찰재체계통적세포독성，도치형광현미경관찰세포형태급록색형광단백표체강도，류식세포의검측세포전염솔。<br> 결과여결론：량충재체분별전염후，라선신경절세포형태균미발생명현개변，단량조재체대라선신경절세포균유일정적독성，실험조재체독성교소차전염효솔교고(P<0.05)。결과설명협산취을이순접지지화취을희아알기인전염체외라선신경절세포적전염효솔급세포독성균우우전통적양리자지질체。
BACKGROUND:A new kind of polyethyenimine-polyethylene glycol (PEI-PEG) is apt to synthesis and modification, and has good stability, easily regulated structure and properties, which has been applied to a variety of diseases as gene transfer vectors. <br> OBJECTIVE:To study the feasibility and the characteristics of nanoparticles PEI-PEG as gene transfer vector for spiral ganglion cells of the inner ear of mice. <br> METHODS:Using PEI-PEG (experimental group) and lipofectamineTM 2000 (control group) as gene transfer vectors, with enhanced green fluorescent protein (EGFP) as tracing protein, spiral ganglion cells were transfected in vitro, the transfection rate and mean fluorescence strength were detected by fluorescence microscopy and flow cytometry. Toxicity effects of each vector on spiral ganglion cells were determined by spectroscopic measurement of MTT method. <br> RESULTS AND CONCLUSION:After transfection with two vectors, spiral ganglion cells had no changes in morphology. The transfection rate of PEI-PEG was statistical y higher than that of liposome (P<0.05). Also, the toxicity effects of PEI-PEG to spiral ganglion cells was lightly than that of liposome (P<0.05). As a new gene transfer vector, PEI-PEG has a higher transfection rate and lower toxicity effects to spiral ganglion cells compared to liposome, and can serve as gene transferring system into cochlear nervous system.
