中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
21期
3341-3344
,共4页
生物材料%骨生物材料%聚乙稀吡咯啉酮%骨形态发生蛋白%纳米晶胶原基骨%缓释
生物材料%骨生物材料%聚乙稀吡咯啉酮%骨形態髮生蛋白%納米晶膠原基骨%緩釋
생물재료%골생물재료%취을희필각람동%골형태발생단백%납미정효원기골%완석
biocompatible materials%bone morphogenetic proteins%brace%delayed-action preparations
背景：临床试验证明纳米晶胶原基骨支架材料具有较好的组织相容性、合适的孔隙率及降解性能，但缺乏缓释生长因子的作用，且无成骨诱导性。<br> 目的：将聚乙稀吡咯啉酮与骨形态发生蛋白复合后形成复合颗粒，再修饰纳米晶胶原基骨制备复合支架，观察骨形态发生蛋白的体外缓释效果。<br> 方法：实验分3组，实验组取冻存管2支，每支放入5 mm×5 mm×5 mm 纳米晶胶原基骨1块，滴加聚乙稀吡咯啉酮混匀，-4℃冻存过夜，再滴加骨形态发生蛋白溶液，真空抽干后冻存；对照组1取冻存管2支，每支放入5 mm×5 mm×5 mm 纳米晶胶原基骨1块，滴加骨形态发生蛋白溶液，真空抽干后冻存；对照组2取冻存管2支，每支放入5 mm×5 mm×5 mm未脱钙的大鼠松质骨1块，滴加聚乙稀吡咯啉酮混匀，-4℃冻存过夜，再滴加骨形态发生蛋白溶液，真空抽干后冻存。观察14 d，采用ELISA法检测3组复合物骨形态发生蛋白的体外释放活性。<br> 结果与结论：观察到14 d时，两对照组骨形态发生蛋白A值趋近于0，而实验组上清液中骨形态发生蛋白仍保持较高的A值，组间比较差异有显著性意义(P<0.05)。表明经聚乙烯吡咯烷酮修饰后纳米晶胶原基骨支架具有明显缓释骨形态发生蛋白的作用。
揹景：臨床試驗證明納米晶膠原基骨支架材料具有較好的組織相容性、閤適的孔隙率及降解性能，但缺乏緩釋生長因子的作用，且無成骨誘導性。<br> 目的：將聚乙稀吡咯啉酮與骨形態髮生蛋白複閤後形成複閤顆粒，再脩飾納米晶膠原基骨製備複閤支架，觀察骨形態髮生蛋白的體外緩釋效果。<br> 方法：實驗分3組，實驗組取凍存管2支，每支放入5 mm×5 mm×5 mm 納米晶膠原基骨1塊，滴加聚乙稀吡咯啉酮混勻，-4℃凍存過夜，再滴加骨形態髮生蛋白溶液，真空抽榦後凍存；對照組1取凍存管2支，每支放入5 mm×5 mm×5 mm 納米晶膠原基骨1塊，滴加骨形態髮生蛋白溶液，真空抽榦後凍存；對照組2取凍存管2支，每支放入5 mm×5 mm×5 mm未脫鈣的大鼠鬆質骨1塊，滴加聚乙稀吡咯啉酮混勻，-4℃凍存過夜，再滴加骨形態髮生蛋白溶液，真空抽榦後凍存。觀察14 d，採用ELISA法檢測3組複閤物骨形態髮生蛋白的體外釋放活性。<br> 結果與結論：觀察到14 d時，兩對照組骨形態髮生蛋白A值趨近于0，而實驗組上清液中骨形態髮生蛋白仍保持較高的A值，組間比較差異有顯著性意義(P<0.05)。錶明經聚乙烯吡咯烷酮脩飾後納米晶膠原基骨支架具有明顯緩釋骨形態髮生蛋白的作用。
배경：림상시험증명납미정효원기골지가재료구유교호적조직상용성、합괄적공극솔급강해성능，단결핍완석생장인자적작용，차무성골유도성。<br> 목적：장취을희필각람동여골형태발생단백복합후형성복합과립，재수식납미정효원기골제비복합지가，관찰골형태발생단백적체외완석효과。<br> 방법：실험분3조，실험조취동존관2지，매지방입5 mm×5 mm×5 mm 납미정효원기골1괴，적가취을희필각람동혼균，-4℃동존과야，재적가골형태발생단백용액，진공추간후동존；대조조1취동존관2지，매지방입5 mm×5 mm×5 mm 납미정효원기골1괴，적가골형태발생단백용액，진공추간후동존；대조조2취동존관2지，매지방입5 mm×5 mm×5 mm미탈개적대서송질골1괴，적가취을희필각람동혼균，-4℃동존과야，재적가골형태발생단백용액，진공추간후동존。관찰14 d，채용ELISA법검측3조복합물골형태발생단백적체외석방활성。<br> 결과여결론：관찰도14 d시，량대조조골형태발생단백A치추근우0，이실험조상청액중골형태발생단백잉보지교고적A치，조간비교차이유현저성의의(P<0.05)。표명경취을희필각완동수식후납미정효원기골지가구유명현완석골형태발생단백적작용。
BACKGROUND:Clinical trials have proved that nano-hydroxyapatite/col agen bone scaffold has good tissue compatibility, suitable porosity and degradation performance, but it is limited by the lack of sustained-release growth factors and induced osteogenic capacity. <br> OBJECTIVE:To study the effect of the sustained-release system of polyethylene pyrrolinone modified nano-hydroxyapatite composite scaffold carrying bone morphogenetic protein. <br> METHODS:Samples were divided into three groups, and there were two cryogenic vials in each group. In the experimental group, a piece of 5 mm×5 mm×5 mm nano-hydroxyapatite/col agen bone was placed per vial and mixed with polyethylene pyrrolinone at-4℃overnight, and bone morphogenetic protein solution was then added fol owed by vacuum pumping and cryopreservation. In the control group 1, a piece of 5 mm×5 mm×5 mm nano-hydroxyapatite/col agen bone was placed per vial and mixed with bone morphogenetic protein solution fol owed by vacuum pumping and cryopreservation. In the control group 2, a piece of 5 mm×5 mm×5 mm non-decalcified rat cancellous bone was placed per vial and mixed with polyethylene pyrrolinone at-4℃overnight, and bone morphogenetic protein solution was then added fol owed by vacuum pumping and cryopreservation. After 14 days, the in vitro release activity of bone morphogenetic protein in the three groups was detected using enzyme-linked immunosorbent assay. <br> RESULTS AND CONCLUSION:After 14 days, the absorbance values of bone morphogenetic protein in the two control groups were close to 0, whereas the experimental group showed a higher absorbance value (P<0.05). It indicates that polyethylene pyrrolinone modified bone morphogenetic protein/nano-hydroxyapatite composite scaffold presents good sustained-release effects.
