CAJ | 학술논문

目的：探讨不同剂量乙醛腹腔注射后对大鼠心肌细胞线粒体琥珀酸脱氢酶（ SDH）活性的影响，并在透射电镜下观察大鼠心肌细胞线粒体形态，进一步探索乙醛心肌损害的可能机制。方法将健康Wistar大鼠65只按体质量随机分为4组：正常对照组20只、乙醛低剂量组15只、乙醛高剂量组15只及左卡尼汀组15只。给药4周时透射电镜下观察心肌线粒体形态，生化反应方法检测线粒体SDH活性、血浆超氧化物歧化酶（ SOD）及血浆丙二醛（ MDA）的含量。结果透射电镜下可见心肌线粒体出现水肿、线粒体脊紊乱、嵴消失以及线粒体膜缺失等改变，与对照组比较差异显著。左卡尼汀组的心肌线粒体SDH活性及血浆SOD含量较乙醛高剂量组升高（ P均＜0．05），MDA含量较乙醛高剂量组降低（P＜0．05）。结论乙醛腹腔注射液可损伤大鼠心肌细胞，心肌线粒体损伤明显；氧化应激参与了乙醛的心肌损害过程，左卡尼汀可以减轻乙醛的心肌损害。
목적：탐토불동제량을철복강주사후대대서심기세포선립체호박산탈경매（ SDH）활성적영향，병재투사전경하관찰대서심기세포선립체형태，진일보탐색을철심기손해적가능궤제。방법장건강Wistar대서65지안체질량수궤분위4조：정상대조조20지、을철저제량조15지、을철고제량조15지급좌잡니정조15지。급약4주시투사전경하관찰심기선립체형태，생화반응방법검측선립체SDH활성、혈장초양화물기화매（ SOD）급혈장병이철（ MDA）적함량。결과투사전경하가견심기선립체출현수종、선립체척문란、척소실이급선립체막결실등개변，여대조조비교차이현저。좌잡니정조적심기선립체SDH활성급혈장SOD함량교을철고제량조승고（ P균＜0．05），MDA함량교을철고제량조강저（P＜0．05）。결론을철복강주사액가손상대서심기세포，심기선립체손상명현；양화응격삼여료을철적심기손해과정，좌잡니정가이감경을철적심기손해。
Objective To investigate the effects of different dosages of acetaldehyde on the activity variation of myo -cardial mitochondrial succinate dehydrogenase ( SDH) of rats by intraperitoneal injection and observe the histomorphology of myocardial mitochondria by transmission electron microscope ( TEM) in order to further discover the possible mechanism of myocardium injury by ethanol .Methods Sixty-five Wistar rats were randomly divided into four groups:the control group (n=20, group C), low dose acetaldehyde group (n=15, group L), high dose acetaldehyde group (n=15, group H) and levocarnitine group (n=15).Acetaldehyde (110 mg/kg and 220 mg/kg) were injected into the rats′abdominal cavi-ties once a day for 4 weeks .TEM was applied to observe the mitochondrial histomorphology of all groups .SDH activity in the myocardium, superoxide dismutase ( SOD) and malondialdehyde ( MDA) in plasma were detected by biochemical methods .Results Compared with group C , under the TEM , we observed that the mitochondria swelled , and the ridge dis-appeared with incomplete or no capsule in group L , group H and levocarnitine group .The SDH activity of the levocarnitine group was significantly higher than of that of group H (P<0.05) as well as the content of SOD (P<0.05), but the con-tent of MDA was significantly lower than that of group H (P<0.05).Conclusions The intraperitoneal injection of acetal-dehyde could damage the myocardial cells of rats , especially resulting in the myocardial mitochondrial impairment .Oxida-tive stress may take part in the process of myocardial injuries , and levocarnitine may reduce the myocardial damage of acet-aldehyde .