CAJ | 학술논문

目的：探讨早期低剂量接触氯化甲基汞（ Methyl Mercury Chloride ， MMC）对大鼠生殖功能和生长发育的影响以及枸杞多糖（ Lycium Barbarum Polysaccharides ，LBP）的早期干预。方法：将60只健康7日龄大鼠随机分为对照组（ CON）、MMC组、MMC＋LBP组、LBP组，MMC组和MMC＋LBP组均颈部皮下注射MMC，CON组颈部注射相同剂量的生理盐水， MMC＋LBP组同时喂养LBP，雌雄合笼，自然观察各组体重发育以及生殖繁育能力以及子代仔鼠早期生长发育和存活状况。再将已孕母鼠哺乳期仔鼠染毒，观察母鼠产仔情况。结果：1）MMC组毛色萎缩，精神状态倦怠、动作不活泼、食欲不振。2）MMC组生长较缓慢且随染毒时间的延长而明显。3）MMC组染毒大鼠未产仔，MMC＋LBP组有产仔，但产死率高、出生后存活率低于CON；LBP组产死率较CON低。4）MMC＋LBP组有畸胎，且仔鼠出生体重与正常CON和LBP组仔鼠出生体重有统计学意义（P＜0.01）。5）染毒仔鼠影响其已孕母鼠再次生育。结论：MMC对大鼠有生殖发育毒性，LBP可抑制MMC引起的生殖毒性。
목적：탐토조기저제량접촉록화갑기홍（ Methyl Mercury Chloride ， MMC）대대서생식공능화생장발육적영향이급구기다당（ Lycium Barbarum Polysaccharides ，LBP）적조기간예。방법：장60지건강7일령대서수궤분위대조조（ CON）、MMC조、MMC＋LBP조、LBP조，MMC조화MMC＋LBP조균경부피하주사MMC，CON조경부주사상동제량적생리염수， MMC＋LBP조동시위양LBP，자웅합롱，자연관찰각조체중발육이급생식번육능력이급자대자서조기생장발육화존활상황。재장이잉모서포유기자서염독，관찰모서산자정황。결과：1）MMC조모색위축，정신상태권태、동작불활발、식욕불진。2）MMC조생장교완만차수염독시간적연장이명현。3）MMC조염독대서미산자，MMC＋LBP조유산자，단산사솔고、출생후존활솔저우CON；LBP조산사솔교CON저。4）MMC＋LBP조유기태，차자서출생체중여정상CON화LBP조자서출생체중유통계학의의（P＜0.01）。5）염독자서영향기이잉모서재차생육。결론：MMC대대서유생식발육독성，LBP가억제MMC인기적생식독성。
Objective:To detect the toxicity of neonatal exposed to methylmercury chloride on the adulthood reproduction ,as well as ear-ly growth and development in rats and the intervention of Lycium barbarum polysaccharide .Methods:Sixty SD of 7 d rats were randomly divided into saline Control , MMC, MMC+LBP, LBP.The natural development , weight growth and fertilities were observed .Healthy pregnant female rats and MMC exposed neonatal rats and were also observed .Results:1) Compared with the control group , the rats in MMC group was dropping, inactivity, loss of appetite;2) The weights of rats in MMC group grew slower than in control rats with the ex-posure time dependently;3) MMC group were infertility after exposure to the methyl mercury .There remained offspring in MMC+LBP group and their after-born death rates were higher than that of the control group , with low birth weights and low survival rates;4 ) The weights in MMC+LBP rats were significant lower (P<0.01) than that in in control and LBP group;5) The MMC exposed pups influ-enced the next reproduction of the female rats .Conclusion:Methylmercury chloride has development and reproductive toxicity effects on rats.Lycium barbarum polysaccharides have potential protection against the toxicity roles .