CAJ | 학술논문

目的：本实验采用可注射骨修复材料（ Injectable Bone Regeneration Composite ，IBRC）复合脂肪间充质干细胞（ Adipose Tis-sue-Derived Mesenchymal Stromal cells ，ADMSCs）应用于兔腰椎横突间融合模型，骨碎补总黄酮（ Assemble Flavone of Drynaria Rhizome，AFDR）灌胃，观察融合效果。方法：60只成年白兔，制成兔腰椎横突间融合模型，采用安全随机法随机分为5组：A组植入IBRC材料结合AFDR灌胃；B组植入IBRC材料复合自体髂骨（ Autologous iliac Crest Bone ，ACB），结合去离子水﹙Deion-ized Water，DW﹚灌胃；C组植入IBRC材料＋ADMSCs，DW灌胃；D组，植入ACB，DW灌胃；E组植入ACB，AFDR灌胃。以术后第8周为观察时间点，进行大体观测，手触检测，X线片，组织学观察。结果：IRBC＋ADMSCs，ACB＋AFDR组获得较好的融合率，显著高于单纯IRBC，ACB组（ P＜0.05），与ACB＋DW组相比差异无统计学意义（ P＞0.05）。结论：自体ADMSCs结合IRBC材料可实现与ACB组相似的融合率，结合中药AFDR灌胃，能够提高兔腰椎横突间的骨性融合率。
목적：본실험채용가주사골수복재료（ Injectable Bone Regeneration Composite ，IBRC）복합지방간충질간세포（ Adipose Tis-sue-Derived Mesenchymal Stromal cells ，ADMSCs）응용우토요추횡돌간융합모형，골쇄보총황동（ Assemble Flavone of Drynaria Rhizome，AFDR）관위，관찰융합효과。방법：60지성년백토，제성토요추횡돌간융합모형，채용안전수궤법수궤분위5조：A조식입IBRC재료결합AFDR관위；B조식입IBRC재료복합자체가골（ Autologous iliac Crest Bone ，ACB），결합거리자수﹙Deion-ized Water，DW﹚관위；C조식입IBRC재료＋ADMSCs，DW관위；D조，식입ACB，DW관위；E조식입ACB，AFDR관위。이술후제8주위관찰시간점，진행대체관측，수촉검측，X선편，조직학관찰。결과：IRBC＋ADMSCs，ACB＋AFDR조획득교호적융합솔，현저고우단순IRBC，ACB조（ P＜0.05），여ACB＋DW조상비차이무통계학의의（ P＞0.05）。결론：자체ADMSCs결합IRBC재료가실현여ACB조상사적융합솔，결합중약AFDR관위，능구제고토요추횡돌간적골성융합솔。
Objective:To explore the effect of Adipose Tissue-Derived Mesenchymal Stromal cells ( ADMSCs) which is Injectable Bone Regeneration Composite ( IBRC) combined with intra-gastric administration of Assemble Flavone of Drynaria Rhizome ( AFDR) on rab-bits with lumbar transverse interbody fusion .Methods:Sixty adult rabbits were made into rabbit lumbar transverse interbody fusion mod-els,randomly divided into five groups including A group (IBRC+AFDR),B group (IBRC+Autologous iliac Crest Bone(ACB) +Dei-onized Water ( DW) ) ,C group ( IBRC+ADMSCs+DW) ,D group ( ACB+DW) ,E group ( ACB +AFDR) .After eight weeks of treat-ment,specimens were collected for gross observation ,touch detection,X-ray,histological observation.Results:The fusion rates of group C and group E were significantly higher than that of group B (P<0.05),and there was no significantly statistical difference (P>0.05) compared with the group D .Conclusion:IRBC composite ADMSCs can achieve a similar fusion rate as ACB group .Combing with tradi-tional Chinese AFDR can improve the rate of the intertransverse lumbar fusion in rabbits .