CAJ | 학술논문

目的：研究缺血性心力衰竭气虚血瘀证和阳虚水停证的血浆代谢组学特征，探索代谢组学在缺血性心力衰竭中医证候中的应用。方法：收集缺血性心力衰竭气虚血瘀证和阳虚水停证患者各18例，利用液相色谱质谱（ LC/MS）联用技术对样本进行检测，用主成分分析（ PCA）及偏最小二乘分析判别法（ PLS-DA）处理图谱数据，研究两组血浆代谢物图谱的差异。结果：两组患者血浆LC-MS图谱的PCA及PLS-DA分析显示，气虚血瘀证和阳虚水停证能够被明显区分，两组血浆代谢物含量存在明显差异。缺血性心力衰竭阳虚水停证较气虚血瘀证，3种溶血磷脂酰胆碱类物质含量降低，3种肉碱类物质含量升高，2中脂肪酸类物质含量升高，肌酐含量升高。结论：缺血性心力衰竭阳虚水停证和气虚血瘀证具有不同的代谢模式，两组能量代谢及磷脂代谢存在差异。以LC-MS为技术特点的代谢组学研究方法能够将两者区分，为中医证候的客观诊断提供了新的检测方法。
목적：연구결혈성심력쇠갈기허혈어증화양허수정증적혈장대사조학특정，탐색대사조학재결혈성심력쇠갈중의증후중적응용。방법：수집결혈성심력쇠갈기허혈어증화양허수정증환자각18례，이용액상색보질보（ LC/MS）련용기술대양본진행검측，용주성분분석（ PCA）급편최소이승분석판별법（ PLS-DA）처리도보수거，연구량조혈장대사물도보적차이。결과：량조환자혈장LC-MS도보적PCA급PLS-DA분석현시，기허혈어증화양허수정증능구피명현구분，량조혈장대사물함량존재명현차이。결혈성심력쇠갈양허수정증교기허혈어증，3충용혈린지선담감류물질함량강저，3충육감류물질함량승고，2중지방산류물질함량승고，기항함량승고。결론：결혈성심력쇠갈양허수정증화기허혈어증구유불동적대사모식，량조능량대사급린지대사존재차이。이LC-MS위기술특점적대사조학연구방법능구장량자구분，위중의증후적객관진단제공료신적검측방법。
Objective:To investigate metabolomics characteristics of serum samples in ischemia heart failure with syndrome of blood sta -sis caused by qi deficiency ( QDBS) and syndrome of fluid accumulation caused by Yang Deficiency ( YDFA) and explore the applica-tion of metabolomics in study of TCM syndrome of ischemia heart failure .Methods:The serum samples of ischemia heart failure with QDBS (n=18) and YDFA (n=18) were detected by Liquid chromatography-mass spectrometry(LC-MS) and analyzed based on Prin-cipal Component Analysis ( PCA) and Partial Least Squares Discriminant Analysis ( PLS-DA) .Results:Ischemia heart failure with QDBS and YDFA can be distinguished clearly by PLS-DA.There was significant difference in metabolic characteristic between the two groups . Compared with QDBS ,3 metabolites belonged to Lysophosphatidylcholines decreased in YDFA;3 metabolites belonged to carnitines and 2 metabolites belonged to fatty and creatinine increased in YDFA .Conclusion:Energy metabolism and phospholipid metabolism were different between ischemia heart failure with QDBS and YDFA .Metabolomics analysis based on LC/MS can distinguish the two groups and provide new detective method for TCM syndromes differentiation objective .