水产科学
水產科學
수산과학
FISHERIES SCIENCE
2013年
12期
717-720
,共4页
祝尧荣%沈文英%金叶飞%任岗
祝堯榮%瀋文英%金葉飛%任崗
축요영%침문영%금협비%임강
异育银鲫%饥饿%胰岛素%甲状腺激素%生化组分
異育銀鯽%饑餓%胰島素%甲狀腺激素%生化組分
이육은즉%기아%이도소%갑상선격소%생화조분
Carassius auratus gibelio%starvation%triiodothyronine%insulin%approximate composition
选取体质量(82.1±4.5) g的健康异育银鲫120尾,随机分成对照组和处理组,每组3个平行,每个平行20尾鱼。对照组正常投喂,处理组饥饿处理,试验期为28 d ,养殖温度22~25℃。饥饿3、7、14、21、28 d后,分别测定异育银鲫血液中甲状腺素、胰岛素水平及肝胰脏、肌肉生化组分的含量。试验结果表明,饥饿降低了异育银鲫血液甲状腺激素和胰岛素含量,其中饥饿处理7 d和21 d组异育银鲫血液中甲状腺激素含量显著低于对照组( P<0.05);饥饿7、21、28 d ,胰岛素与对照组差异显著( P<0.05)。饥饿3、7、14 d的异育银鲫肝糖原和肌糖原含量显著降低( P<0.05),肝胰脏和肌肉脂肪含量在整个饥饿过程中均显著低于对照组(P<0.05),饥饿对肝胰脏和肌肉的蛋白含量和水分无显著影响。因此饥饿降低了异育银鲫甲状腺激素和胰岛素水平及代谢水平,可能是鱼体应对饥饿胁迫的重要生理反应。
選取體質量(82.1±4.5) g的健康異育銀鯽120尾,隨機分成對照組和處理組,每組3箇平行,每箇平行20尾魚。對照組正常投餵,處理組饑餓處理,試驗期為28 d ,養殖溫度22~25℃。饑餓3、7、14、21、28 d後,分彆測定異育銀鯽血液中甲狀腺素、胰島素水平及肝胰髒、肌肉生化組分的含量。試驗結果錶明,饑餓降低瞭異育銀鯽血液甲狀腺激素和胰島素含量,其中饑餓處理7 d和21 d組異育銀鯽血液中甲狀腺激素含量顯著低于對照組( P<0.05);饑餓7、21、28 d ,胰島素與對照組差異顯著( P<0.05)。饑餓3、7、14 d的異育銀鯽肝糖原和肌糖原含量顯著降低( P<0.05),肝胰髒和肌肉脂肪含量在整箇饑餓過程中均顯著低于對照組(P<0.05),饑餓對肝胰髒和肌肉的蛋白含量和水分無顯著影響。因此饑餓降低瞭異育銀鯽甲狀腺激素和胰島素水平及代謝水平,可能是魚體應對饑餓脅迫的重要生理反應。
선취체질량(82.1±4.5) g적건강이육은즉120미,수궤분성대조조화처리조,매조3개평행,매개평행20미어。대조조정상투위,처리조기아처리,시험기위28 d ,양식온도22~25℃。기아3、7、14、21、28 d후,분별측정이육은즉혈액중갑상선소、이도소수평급간이장、기육생화조분적함량。시험결과표명,기아강저료이육은즉혈액갑상선격소화이도소함량,기중기아처리7 d화21 d조이육은즉혈액중갑상선격소함량현저저우대조조( P<0.05);기아7、21、28 d ,이도소여대조조차이현저( P<0.05)。기아3、7、14 d적이육은즉간당원화기당원함량현저강저( P<0.05),간이장화기육지방함량재정개기아과정중균현저저우대조조(P<0.05),기아대간이장화기육적단백함량화수분무현저영향。인차기아강저료이육은즉갑상선격소화이도소수평급대사수평,가능시어체응대기아협박적중요생리반응。
The levels of triiodothyronine (T3 ) and insulin (INS) in serum and approximate composition in hepatopancreas and muscle were determined in crucian carp Carassius auratus gibelio with initial body weight of(82 .1 ± 4 .5) g starved for 3 ,7 ,14 and 28 days as treatments and fed as control with triplication (20 fish per group ,total of 120 fish) at water temperature of 22~25 ℃ .The results showed that the level of serum T3 was found significant decrease 7 , and 21d after starvation , and the level of serum INS significant decrease 7 ,21 ,28 d after starvation (P< 0 .05) .There were significantly lower glycogen contents in hepatopancreas and muscle 3 ,7 ,and 14 d after starvation than those in the control group (P<0 .05) .There was no significant difference in protein level and moisture in hepatopancreas and muscle in the fed fish and starved fish .The lipid contents in hepatopancreas and muscle were found to be rapidly decreased during starvation (P< 0 .05) .The reduction in serum T3 and INS levels was responsible for reduction in the metabolic rate w hich is the physiological responses for the fish to adapt the stress of starvation .