CAJ | 학술논문

为探讨条斑紫菜叶状体细胞对3种抗生素的敏感性，以确定条斑紫菜遗传转化后代适宜的抗性选择质量浓度，将叶状体用海螺酶解离获得原生质体，在培养皿中20℃培养3 d后，在培养液中分别加入G418（0、10、50、100、200、300 mg/L）、卡那霉素（0、50、100、300、500、800 mg/L）或氯霉素（0、5、15、35、65、100 mg/L ），观察细胞分裂与死亡率、小紫菜比率，进行显著性检验。试验结果表明，条斑紫菜叶状体细胞对3种抗生素的敏感性存在显著差异，对抗生素的敏感性为氯霉素＞卡那霉素＞ G418。在条斑紫菜叶状体遗传转化用G418、卡那霉素或氯霉素作抗性筛选时，筛选质量浓度分别为300、50 mg/L及15～35 mg/L。
위탐토조반자채협상체세포대3충항생소적민감성，이학정조반자채유전전화후대괄의적항성선택질량농도，장협상체용해라매해리획득원생질체，재배양명중20℃배양3 d후，재배양액중분별가입G418（0、10、50、100、200、300 mg/L）、잡나매소（0、50、100、300、500、800 mg/L）혹록매소（0、5、15、35、65、100 mg/L ），관찰세포분렬여사망솔、소자채비솔，진행현저성검험。시험결과표명，조반자채협상체세포대3충항생소적민감성존재현저차이，대항생소적민감성위록매소＞잡나매소＞ G418。재조반자채협상체유전전화용G418、잡나매소혹록매소작항성사선시，사선질량농도분별위300、50 mg/L급15～35 mg/L。
The susceptibility of somatic cells of thalli of laver Porphyra yezoensis to three antibiotics was studied in order to screen reliable selection concentrations for recovery of transformation offsprings of the laver .The protoplasts isolated from thalli by seashell enzymes were cultured in medium at 20 ℃ for 3 days , and then transferred to medium with G418(0 ,10 ,50 ,100 ,200 ,and 300 g/L) ,kanamycin(0 ,50 ,100 , 300 ,500 ,and 800 mg/L) or chloramphenicol (0 ,5 ,15 ,35 ,65 ,and 100 mg/L) .The rate of cell division , mortality ,and rate of thallus formation were observed with significant difference test .The results showed that there were significant differences in susceptibility of the somatic cells to three antibiotics at various concentrations .It was found that the susceptibility of the cells from the thalli to the antibiotics was arranged as chloramphenicol > kanamycin > G418 .As selective reagents for recovery of transformation offspring of the thallus ,the concentration was selected a 300 mg/L for G418 ,50 mg/L for kanamycin and 15~35 mg/L for chloramphenicol .