CAJ | 학술논문

本研究2012年底从辽宁省某屠宰场猪鼻咽拭子样品中分离到1株流感病毒，经HA-HI试验和RT-PCR鉴定为H1N1亚型猪流感病毒株，命名为A/swine/Liaoning/01/2012（H1N1），通过对病毒的8个基因片段克隆并测序，并利用分子生物学软件进行遗传演化分析。结果表明，分离株HA基因裂解位点附近的氨基酸序列为IPSIQSR↓G，符合低致病力流感病毒的分子特征。全基因组进化树结果表明，分离株的8个基因片段与A/swine/Jiangsu/40/2011（H1N1）株核苷酸同源性最高，分离株处在类禽型H1N1亚型遗传进化分支上；由于类禽型H1N1猪流感病毒具有潜在感染人的潜力，在国外和国内均有感染人的报道，因此，辽宁省首次分离到该型猪流感病毒对全省养猪业和公共卫生安全具有重要意义，值得深入研究。
본연구2012년저종요녕성모도재장저비인식자양품중분리도1주류감병독，경HA-HI시험화RT-PCR감정위H1N1아형저류감병독주，명명위A/swine/Liaoning/01/2012（H1N1），통과대병독적8개기인편단극륭병측서，병이용분자생물학연건진행유전연화분석。결과표명，분리주HA기인렬해위점부근적안기산서렬위IPSIQSR↓G，부합저치병력류감병독적분자특정。전기인조진화수결과표명，분리주적8개기인편단여A/swine/Jiangsu/40/2011（H1N1）주핵감산동원성최고，분리주처재류금형H1N1아형유전진화분지상；유우류금형H1N1저류감병독구유잠재감염인적잠력，재국외화국내균유감염인적보도，인차，요녕성수차분리도해형저류감병독대전성양저업화공공위생안전구유중요의의，치득심입연구。
In our study, we isolated a swine influenza virus from pigs in an abattoir in Liaoning province. The virus was identified as H1N1subtype by HA-HI test and RT-PCR, and named A/swine/Liaoning/01/2012 (H1N1). All of the cDNAs of the eight vi-ral RNA segments were amplified by RT-PCR, sequenced, analyzed using the DNAStar soft-ware. The amino acid sequence of the HA gene near the cleavage site was IPSIQSR↓G, which was consistent with that of low pathogenic influenza virus. Genomic sequence analysis showed that all of the eight gene segments of A/swine/Liaoning/01/2012 (H1N1)had the highest homologous with the A/swine/Jiangsu/40/2011(H1N1)strain. Phy-logenetic analysis showed that the isolate was in the European avian-like SIV lin-eage. Since human infections with avian-like SIV has been reported worldwide, the iso-lation of Sw/LN/01/12 for the first time in Liaoning province has an important value for pig industry and public health, and further research of the virus is underway..