CAJ | 학술논문

为了探究与老鹳草相关类型花粉花芽的样品电镜制备，采用直接观察法和戊二醛固定冷冻干燥法对青岛老鹳草花粉扫描电镜制样条件进行了研究。结果表明：直接观察法制备的青岛老鹳草花粉颗粒在扫描电镜下形态饱满，结构清晰，花粉表面的纹饰和突起微观结构清晰完整；改进的FA A单固定冷冻干燥法处理的青岛老鹳草花芽形态学结构完整，层次丰富，立体感强，细胞失水皱缩得到明显改善。通过对不同制样方法进行比较，最终得到可靠简单易行的样品制备方法，对牻牛儿苗科植物的研究及相关类型花粉花芽的样品制备有重要的指导意义。
위료탐구여로관초상관류형화분화아적양품전경제비，채용직접관찰법화무이철고정냉동간조법대청도로관초화분소묘전경제양조건진행료연구。결과표명：직접관찰법제비적청도로관초화분과립재소묘전경하형태포만，결구청석，화분표면적문식화돌기미관결구청석완정；개진적FA A단고정냉동간조법처리적청도로관초화아형태학결구완정，층차봉부，입체감강，세포실수추축득도명현개선。통과대불동제양방법진행비교，최종득도가고간단역행적양품제비방법，대방우인묘과식물적연구급상관류형화분화아적양품제비유중요적지도의의。
In order to explore the revelant types of Geranium pollen and flower buds preparation methods of scanning electron microscope ,air-dried and glutaric aldehyde fixed methods were compared to study preparation condition of scanning electron microscopy .The results showed that Geranium pollen grains prepared through direct observation were full and clear in the scanning electron microscopy ,microscopic structure were clear and morphological integrity ;micro-structure of flower bud treated with FAA single fixation were morphological in-tegrity ,layers rich ,stereo sense strong ,cell dehydration shrinkage was improved significantly .Through the comparison of different specimen preparation methods ,reliable technique which was easier in operation and low cost was achieved ,it had important guiding significance for the preparation of Geranium tsingtauense .pollen and bud .