CAJ | 학술논문

为了建立球根秋海棠组培快繁体系，以球根秋海棠（Begonia tuberhybrida Voss）品种金正日的叶为外植体，研究了培养中各因素对叶基部的影响。结果表明：不同叶片部位外植体的诱导和分化能力不同，叶基部愈伤组织诱导和分化能力最强，诱导率和分化率分别为85．7％和85．4％；暗培养和接种前4℃低温预处理降低了外植体的诱导率和分化率，在金正日花离体培养中不宜采用；叶基部最适宜的诱导和分化培养基为MS＋2，4-D 1．0 mg·L-1＋BA 2．0 mg·L-1＋NAA 0．5 mg·L-1。
위료건립구근추해당조배쾌번체계，이구근추해당（Begonia tuberhybrida Voss）품충금정일적협위외식체，연구료배양중각인소대협기부적영향。결과표명：불동협편부위외식체적유도화분화능력불동，협기부유상조직유도화분화능력최강，유도솔화분화솔분별위85．7％화85．4％；암배양화접충전4℃저온예처리강저료외식체적유도솔화분화솔，재금정일화리체배양중불의채용；협기부최괄의적유도화분화배양기위MS＋2，4-D 1．0 mg·L-1＋BA 2．0 mg·L-1＋NAA 0．5 mg·L-1。
In order to establish rapid propagation system of tissue culture for Begonia tuberhybrida Voss ,the leaves of Begonia tuberhybrida Voss cv Jinzhengri were used as explants for regeneration in vitro ,influencing factors of cultivation in vitro were studied .The results showed that the rates of induction and differentiation of four kinds of explants were different ,the leaf base had the highest induction and differentiation rates of 85 .7%and 85 .4% ;the induction and differentiation rates reduced after dark culturing and in 4℃ before inoculation , which were not fit to be used in leaf base culture in vitro of Begonia tuberhybrida Voss cv Jinzhengri ;the best medium for induction and differentiation of the leaf base was MS+2 ,4-D 1 .0 mg·L-1 +BA 2 .0 mg·L-1 +NAA 0 .5 mg·L-1 .