CAJ | 학술논문

为中华鲟淡水人工群体驯养与繁殖过程中的养殖水环境因子调控以及精子冷冻保存和建立精子库提供科学依据，进行了中华鲟子一代（Acipenser sinensis）精子在Na＋、K＋、Ca2＋、Mg2＋和葡萄糖溶液中的活力测定。结果表明：精子在纯水中的最高寿命（Life tmie，LT）和快速运动时间（Fast movement time， FT）分别为108．57 s和39．76 s。Na＋浓度为4‰时精子LT和FT均达到最高（263．33 s和45．33 s）。K＋浓度为0．01‰时精子LT最长（132．33 s），最高FT（46．00 s）则出现在K＋浓度为0．008‰时。LT和 FT在Ca2＋浓度为4‰时均达到最高值（130．67 s 和53．67 s）。Mg2＋浓度为3‰时，LT达到最高（117．33 s），FT最高值（27．20 s）出现在Mg2＋浓度为2‰时。LT在葡萄糖浓度为4％时达到最高值282．80 s，FT最高值（55．88 s）出现在浓度为3％时。表明，适量浓度的 Na＋、K＋、Ca2＋和Mg2＋可以延长精子的 LT和 FT。LT和FT在葡萄糖的峰值均较其在 Na＋和 K＋溶液中高，表明精子能有效利用外源性物质延长其运动时间。与中华鲟野生亲本相比，子一代精子的 LT和 FT均有所下降，说明中华鲟子一代精子的质量较野生亲本低。
위중화심담수인공군체순양여번식과정중적양식수배경인자조공이급정자냉동보존화건립정자고제공과학의거，진행료중화심자일대（Acipenser sinensis）정자재Na＋、K＋、Ca2＋、Mg2＋화포도당용액중적활력측정。결과표명：정자재순수중적최고수명（Life tmie，LT）화쾌속운동시간（Fast movement time， FT）분별위108．57 s화39．76 s。Na＋농도위4‰시정자LT화FT균체도최고（263．33 s화45．33 s）。K＋농도위0．01‰시정자LT최장（132．33 s），최고FT（46．00 s）칙출현재K＋농도위0．008‰시。LT화 FT재Ca2＋농도위4‰시균체도최고치（130．67 s 화53．67 s）。Mg2＋농도위3‰시，LT체도최고（117．33 s），FT최고치（27．20 s）출현재Mg2＋농도위2‰시。LT재포도당농도위4％시체도최고치282．80 s，FT최고치（55．88 s）출현재농도위3％시。표명，괄량농도적 Na＋、K＋、Ca2＋화Mg2＋가이연장정자적 LT화 FT。LT화FT재포도당적봉치균교기재 Na＋화 K＋용액중고，표명정자능유효이용외원성물질연장기운동시간。여중화심야생친본상비，자일대정자적 LT화 FT균유소하강，설명중화심자일대정자적질량교야생친본저。
To provide a scientific basis for the regulation of freshwater aquaculture factors,sperm cryopreservation and sperm bank building,sperm motility of F1-generation of A.sinensis was observed in various concentrations of Na+、K+、Ca2+、Mg2+ and glucose solutions.The results showed that the longest life time (LT)and fast movement time (FT)in water were 108.57 s and 39.76 s respectively.The longest LT (236.33 s)and FT (45.33 s)were realized when the concentration of Na+ was 4‰;when the concentration of K+ was 0.01‰,the longest LT was realized (132.33 s),while the longest FT was realized (46.00 s)when K+ was 0.008‰;the LT and FT were the longest (130.67 s and 53.67 s respectively) when Ca2+ was 4‰.The longest LT (117.33 s)was realized in 3‰ Mg2+ solution,while the longest FT (27.20 s)was realized in 2‰ Mg2+ solution;the longest LT was realized (282.80 s)when the concentration of glucose was 4% and the longest FT was 55.88 s when the concentration was 3%.It indicated that the optimal concentration of Na+ and K+ could increase the LT and FT.Both LT and FT in respective optimal glucose were longer than those in Na+ and K+ solution,which indicated that the sperm could effectively use the exogenous materials to increase LT and FT.Compared with wild parents of Chinese sturgeon,LT and FT of F1-generation were both lower,which indicated that sperm qualitiy of F1-generation was lower than that of wild parents.