CAJ | 학술논문

为弄清菌株 CB3对毒死蜱的降解特性，采用摇床振荡培养等方法，对从长期受有机磷农药污染的土壤和废水中分离出的1株毒死蜱降解菌的降解特性进行了研究。结果表明：CB3生长和对毒死蜱的降解作用具有一定的同步性，当其生长量最大时，对毒死蜱的降解率达89．6％。初始浓度200～800 mg/L时，CB3对毒死蜱的降解率较高；浓度高于800 mg/L 时，随着初始浓度的增加，降解率逐渐下降；当初始浓度为2000 mg/L 时，降解率仅32％。接种量低于1 mL 时，CB3对毒死蜱的降解率随着接种量增加而提高，当接种量在1～2．5 mL 时，CB3对毒死蜱降解率无明显差异。CB3在 pH6．0～7．0，25～35℃条件下对毒死蜱的降解率较高。CB3对有机磷类农药的降解作用具有选择性，利用三唑磷作为碳源时生长良好，利用马拉硫磷和氧乐果作碳源时生长较差，利用辛硫磷和乙酰甲胺磷作为碳源时几乎不生长。
위롱청균주 CB3대독사비적강해특성，채용요상진탕배양등방법，대종장기수유궤린농약오염적토양화폐수중분리출적1주독사비강해균적강해특성진행료연구。결과표명：CB3생장화대독사비적강해작용구유일정적동보성，당기생장량최대시，대독사비적강해솔체89．6％。초시농도200～800 mg/L시，CB3대독사비적강해솔교고；농도고우800 mg/L 시，수착초시농도적증가，강해솔축점하강；당초시농도위2000 mg/L 시，강해솔부32％。접충량저우1 mL 시，CB3대독사비적강해솔수착접충량증가이제고，당접충량재1～2．5 mL 시，CB3대독사비강해솔무명현차이。CB3재 pH6．0～7．0，25～35℃조건하대독사비적강해솔교고。CB3대유궤린류농약적강해작용구유선택성，이용삼서린작위탄원시생장량호，이용마랍류린화양악과작탄원시생장교차，이용신류린화을선갑알린작위탄원시궤호불생장。
The degradation characteristics of CB3,a chlorpyrifos degrading bacteria strain isolated from soil and waste water contaminated by organophosphorus pesticide for long-term was studied.The results showed that there was a certain synchronism between CB3 growth and chlorpyrifos degradation rate and the degradation rate could be up to 89.6% when the growth of CB3 was the maximum.The degradation rate reached a higher level when the initial concentration was 200 ~ 800 mg/L but the degradation rate decreased gradually with increase of initial concentration when the concentration was above 800 mg/L.The degradation rate of the initial concentration with 2 000 mg/L was 32% only.The degradation rate of CB3 to chlorpyrifos rose with increase of inoculation level when the inoculation level was below 1 ml and there was no significant difference in degradation rate when the inoculation level was 1~2.5 ml.The degradation rate of CB3 to chlorpyrifos under pH 6.0~7.0 and 25~35℃ reached a higher level.CB3 grew well on triazophos,grew badly on malathion or omethoate and did not grow on fenthion or acephate,which indicates that CB3 is of the selectivity to the degradation of organophosphorus pesticide.