贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2013年
12期
119-122
,共4页
肖厚军%何佳芳%芶久兰%赵欢%雷正均%颜智华
肖厚軍%何佳芳%芶久蘭%趙歡%雷正均%顏智華
초후군%하가방%구구란%조환%뢰정균%안지화
煤矸石%从枝菌根真菌%草木樨%优势共生体
煤矸石%從枝菌根真菌%草木樨%優勢共生體
매안석%종지균근진균%초목서%우세공생체
coal gangue%arbuscular mycorrhizal fungus%Melilotus albus%dominant symbiote
为筛选出适宜在煤矸石废弃地生物修复所需的丛枝真菌,以煤矸石粉和耕作土壤作供试基质,进行了草木樨接种不同来源从枝菌根真菌(Arbuscular mycorrhizal fungus,AM)的培养试验,研究 AM对草木樨侵染率、促生效应和草木樨对 AM 的依赖度。结果表明:摩西球囊霉(Glomus mosseae,Gm.)和根内球囊霉(Glomus intraradice,Gi.)菌株均能侵染草木樨根部,耕作土壤上的侵染率显著高于煤矸石粉;不同来源菌株的促生效应有差异,且地上部大于根系;草木樨对不同来源菌株的依赖度也有差异,初步认为草木樨与 Gm.-GU(从贵州大学生命科学学院引进的摩西球囊霉)能形成优势共生体。在耕作土壤、煤矸石粉基质上接种 Gm.-GU,菌根侵染率分别为69.7%和21.7%,地上部生物量、根系量、全株生物量和菌根依赖度均高于其他菌株。
為篩選齣適宜在煤矸石廢棄地生物脩複所需的叢枝真菌,以煤矸石粉和耕作土壤作供試基質,進行瞭草木樨接種不同來源從枝菌根真菌(Arbuscular mycorrhizal fungus,AM)的培養試驗,研究 AM對草木樨侵染率、促生效應和草木樨對 AM 的依賴度。結果錶明:摩西毬囊黴(Glomus mosseae,Gm.)和根內毬囊黴(Glomus intraradice,Gi.)菌株均能侵染草木樨根部,耕作土壤上的侵染率顯著高于煤矸石粉;不同來源菌株的促生效應有差異,且地上部大于根繫;草木樨對不同來源菌株的依賴度也有差異,初步認為草木樨與 Gm.-GU(從貴州大學生命科學學院引進的摩西毬囊黴)能形成優勢共生體。在耕作土壤、煤矸石粉基質上接種 Gm.-GU,菌根侵染率分彆為69.7%和21.7%,地上部生物量、根繫量、全株生物量和菌根依賴度均高于其他菌株。
위사선출괄의재매안석폐기지생물수복소수적총지진균,이매안석분화경작토양작공시기질,진행료초목서접충불동래원종지균근진균(Arbuscular mycorrhizal fungus,AM)적배양시험,연구 AM대초목서침염솔、촉생효응화초목서대 AM 적의뢰도。결과표명:마서구낭매(Glomus mosseae,Gm.)화근내구낭매(Glomus intraradice,Gi.)균주균능침염초목서근부,경작토양상적침염솔현저고우매안석분;불동래원균주적촉생효응유차이,차지상부대우근계;초목서대불동래원균주적의뢰도야유차이,초보인위초목서여 Gm.-GU(종귀주대학생명과학학원인진적마서구낭매)능형성우세공생체。재경작토양、매안석분기질상접충 Gm.-GU,균근침염솔분별위69.7%화21.7%,지상부생물량、근계량、전주생물량화균근의뢰도균고우기타균주。
To sift out a few arbuscular mycorrhizal fungus suitable for bioremediation in coal gangue, culture experiments were conducted by inoculating with AM fungus in coal gangue and upland soil to study the effects of AM fungus on infection and growth of M.albus seedlings.The results showed that G. mosseae and G.intraradice could all infect the root of M.albus seedlings. The infection rate was significantly higher in cultivated soil than in coal gangue;The difference of improving the M.albus growth by inoculating with different AM fungus strains was observed,which was bigger in overground part than in the root;The dependence of M.albus to AM fungus was obviously observed.M.albus and Gm.-GU could form dominant symbiont.The infection rates was respectively 69.7% and 21.7% in cultivated soil and coal gangue matrix.The dependence of M.albus to AM fungus of overground biomass,root quantity and complete stool biomass was higher than other strains.