合肥工业大学学报（自然科学版）
閤肥工業大學學報（自然科學版）
합비공업대학학보（자연과학판）
JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE)
2013年
12期
1518-1522
,共5页
柏晓娅%顾菊%陈光朗%孙成磊%阳立波%曹树青
柏曉婭%顧菊%陳光朗%孫成磊%暘立波%曹樹青
백효아%고국%진광랑%손성뢰%양립파%조수청
抗旱%拟南芥%CSM1基因%鉴定%TAIL-PCR技术
抗旱%擬南芥%CSM1基因%鑒定%TAIL-PCR技術
항한%의남개%CSM1기인%감정%TAIL-PCR기술
drought resistance%A rabidopsis%CSM1 gene%identification%TAIL-PCR technology
干旱是植物生长和发育的限制因素之一，因此抗旱基因克隆和功能研究具有重要的理论意义和应用价值。文章利用甘露醇模拟干旱胁迫，以发芽为筛选指标，从拟南芥突变体库中筛选鉴定了抗旱突变体csm1-1。生理生化分析表明，csm1-1抗旱性与其电导率、可溶性糖质量比和脯氨酸积累有关。利用TAIL-PCR技术和RT-PCR分析发现，CSM1基因（RCI3）编码一个过氧化物酶。研究结果为进一步研究该基因调控植物抗旱的机理提供了基础。
榦旱是植物生長和髮育的限製因素之一，因此抗旱基因剋隆和功能研究具有重要的理論意義和應用價值。文章利用甘露醇模擬榦旱脅迫，以髮芽為篩選指標，從擬南芥突變體庫中篩選鑒定瞭抗旱突變體csm1-1。生理生化分析錶明，csm1-1抗旱性與其電導率、可溶性糖質量比和脯氨痠積纍有關。利用TAIL-PCR技術和RT-PCR分析髮現，CSM1基因（RCI3）編碼一箇過氧化物酶。研究結果為進一步研究該基因調控植物抗旱的機理提供瞭基礎。
간한시식물생장화발육적한제인소지일，인차항한기인극륭화공능연구구유중요적이론의의화응용개치。문장이용감로순모의간한협박，이발아위사선지표，종의남개돌변체고중사선감정료항한돌변체csm1-1。생리생화분석표명，csm1-1항한성여기전도솔、가용성당질량비화포안산적루유관。이용TAIL-PCR기술화RT-PCR분석발현，CSM1기인（RCI3）편마일개과양화물매。연구결과위진일보연구해기인조공식물항한적궤리제공료기출。
Drought is one of the limiting factors for plant grow th and development ,and therefore the cloning of novel drought-tolerant genes is very necessary . The wild-type and mutant lines of Arabidopsis were treated with mannitol to simulate drought stress to screen for drought-resistant mu-tants by germination analysis .One drought-tolerant csm1-1 mutant was isolated .The results of physi-ological and biochemical analysis showed that the enhanced drought tolerance of csm1-1 was related with lower conductivity and increased accumulation of soluble sugars and proline .By using TAIL-PCR technology and RT-PCR analysis ,it was found that CSM1 was the RCI3 gene encoding a peroxidase . These results provide a basis for further research on the mechanism of plant drought stress responses .
