CAJ | 학술논문

为了研究马尾松（Pinus massoniana Lamb．）4CL 基因的遗传进化及系统发育情况，根据NCBI 上已登录的4CL 基因的序列，比对分析，利用同源序列设计引物，以马尾松基因组 DNA 为模板，扩增核酸序列，与其他21种松属植物4CL 基因进行 SNP 对比分析。结果表明：扩增出474 bp 核酸序列，松属植物4CL 基因共存在28个 SNP 位点，总核苷酸多样性θw＝0．03483、πT＝0．03621，具较高的多态性。系统进化树聚类结果表明，22个松属植物均集中在第一、二大类，马尾松、火炬松、班克松和小干松聚为第一大类中的第二亚类，亲缘关系较近。
위료연구마미송（Pinus massoniana Lamb．）4CL 기인적유전진화급계통발육정황，근거NCBI 상이등록적4CL 기인적서렬，비대분석，이용동원서렬설계인물，이마미송기인조 DNA 위모판，확증핵산서렬，여기타21충송속식물4CL 기인진행 SNP 대비분석。결과표명：확증출474 bp 핵산서렬，송속식물4CL 기인공존재28개 SNP 위점，총핵감산다양성θw＝0．03483、πT＝0．03621，구교고적다태성。계통진화수취류결과표명，22개송속식물균집중재제일、이대류，마미송、화거송、반극송화소간송취위제일대류중적제이아류，친연관계교근。
To explore the genetic evolution and phylogenetic of 4CL gene family from P .massoniana, the 4CL gene in P .massoniana was cloned,during which,the consensus sequences of previous publish in NCBI were used to design the primers and PCR was performed on genomic DNA of P .massoniana.The results showed that there were 28 single nucleotide polymorphisms (SNP)marker between the 22 4CL gene sequences of genus Pinus.The average nucleotide diversity for the sequenced regions was calculated to beθw=0.034 83 and πT =0.036 21,with higher polymorphism.Alignment of sequences of 4CL gene with MEGA 5.2 indicated that 22 genus Pinus were clustered together to two major groups,showed a low genetic divergence among the populations of 22 genus Pinus in their evolution process,but there was a significant interspecific difference. The closer relationship was for P . massoniana, P . taeda, P .banksiana and P .contorta.The evolution of 4CL gene in the process of plant evolution were relatively conservative,because of extensive distribution of pine,so polymorphism of 4CL gene was high,the divergence should be fully considered in the 4CL gene research.