CAJ | 학술논문

为了研究钾元素对烟草光合作用的影响，以共转化且超量表达烟草钾离子转运体蛋白基因HAK 1的转基因烟草植株（T1-51、T1-87、T1-100和 T1-122）为材料，研究各株系光合特征指标对不同光合有效辐射（PAR）的响应变化规律。结果表明：转基因植株的最大净光合速率皆高于野生型植株；当PAR＜2000μmol/（m2·s）时，各植株的气孔导度和蒸腾速率随 PAR 的增加而增大，且均高于野生型；转基因植株对水分利用率随着 PAR 值升高而增加。光合特征指标测定表明，超量表达 NtHAK 1基因的转基因植株能增加叶片的气孔导度，提高植株的净光合速率及蒸腾速率，进而增强植物的光合效率。
위료연구갑원소대연초광합작용적영향，이공전화차초량표체연초갑리자전운체단백기인HAK 1적전기인연초식주（T1-51、T1-87、T1-100화 T1-122）위재료，연구각주계광합특정지표대불동광합유효복사（PAR）적향응변화규률。결과표명：전기인식주적최대정광합속솔개고우야생형식주；당PAR＜2000μmol/（m2·s）시，각식주적기공도도화증등속솔수 PAR 적증가이증대，차균고우야생형；전기인식주대수분이용솔수착 PAR 치승고이증가。광합특정지표측정표명，초량표체 NtHAK 1기인적전기인식주능증가협편적기공도도，제고식주적정광합속솔급증등속솔，진이증강식물적광합효솔。
In order to study the effects of potassium on photosynthesis,the photosynthetic indexes of the transgenic tobacco plants(line T1-51,line T1-87,line T1-100 and line T1-122)which over expressed the tobacco high-affinity potassium transporter protein 1 gene were studied.The results showed that the photosynthetic indexes,including net photosynthetic rate,stomatal conductance and transpiration rate were higher than those of wild-type tobacco plants while the value of photosynthetically active radiation (PAR)was lower than 2 000 μmol/(m2 · s).Meanwhile,the efficiency of water application raised up following the increment of PAR. We could conclude from these results referred above that the overexpression of NtHAK 1 gene in transgenic tobacco plants could improve their photosynthesis rate.