动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2013年
12期
124-127
,共4页
覃初斌%代盈盈%王文丽%刘红改%赵慧英
覃初斌%代盈盈%王文麗%劉紅改%趙慧英
담초빈%대영영%왕문려%류홍개%조혜영
妊娠期%子宫%G蛋白偶联受体30%奶山羊
妊娠期%子宮%G蛋白偶聯受體30%奶山羊
임신기%자궁%G단백우련수체30%내산양
pregnancy stage%uterus%G protein-coupled receptor 30%dairy goat
为了探讨雌激素膜受体 G 蛋白偶联受体30（G protein-coupled receptor 30，GPR30）在妊娠期山羊子宫组织中的分布和表达变化规律，通过免疫组织化学 SP 法，分别对妊娠前期（1 d～30 d）、妊娠中期（31 d～120 d）和妊娠后期（121 d～150 d）关中奶山羊子宫中 GPR30的分布及相对表达量的变化进行了研究。结果表明，GPR30免疫反应阳性产物在关中奶山羊子宫的子宫内膜、肌层和浆膜均有不同程度分布，主要位于细胞膜和细胞质，其中在子宫内膜腔上皮细胞和腺上皮中阳性细胞最多、着色最深，且随妊娠时期推移逐渐增强；肌层着色细胞染色较浅或无，浆膜层几乎无着色。子宫组织中 GPR30相对表达量呈妊娠前期＜妊娠中期＜妊娠后期的趋势，妊娠早期表达量最低且极显著低于妊娠中期和后期（P ＜0．01），妊娠后期表达量达最高值，但与妊娠中期无显著差别（P ＞0．05）。GPR30在妊娠期关中奶山羊子宫的分布和表达具有一定的规律且主要通过对子宫内膜分泌的调节参与妊娠及分娩等生理活动。
為瞭探討雌激素膜受體 G 蛋白偶聯受體30（G protein-coupled receptor 30，GPR30）在妊娠期山羊子宮組織中的分佈和錶達變化規律，通過免疫組織化學 SP 法，分彆對妊娠前期（1 d～30 d）、妊娠中期（31 d～120 d）和妊娠後期（121 d～150 d）關中奶山羊子宮中 GPR30的分佈及相對錶達量的變化進行瞭研究。結果錶明，GPR30免疫反應暘性產物在關中奶山羊子宮的子宮內膜、肌層和漿膜均有不同程度分佈，主要位于細胞膜和細胞質，其中在子宮內膜腔上皮細胞和腺上皮中暘性細胞最多、著色最深，且隨妊娠時期推移逐漸增彊；肌層著色細胞染色較淺或無，漿膜層幾乎無著色。子宮組織中 GPR30相對錶達量呈妊娠前期＜妊娠中期＜妊娠後期的趨勢，妊娠早期錶達量最低且極顯著低于妊娠中期和後期（P ＜0．01），妊娠後期錶達量達最高值，但與妊娠中期無顯著差彆（P ＞0．05）。GPR30在妊娠期關中奶山羊子宮的分佈和錶達具有一定的規律且主要通過對子宮內膜分泌的調節參與妊娠及分娩等生理活動。
위료탐토자격소막수체 G 단백우련수체30（G protein-coupled receptor 30，GPR30）재임신기산양자궁조직중적분포화표체변화규률，통과면역조직화학 SP 법，분별대임신전기（1 d～30 d）、임신중기（31 d～120 d）화임신후기（121 d～150 d）관중내산양자궁중 GPR30적분포급상대표체량적변화진행료연구。결과표명，GPR30면역반응양성산물재관중내산양자궁적자궁내막、기층화장막균유불동정도분포，주요위우세포막화세포질，기중재자궁내막강상피세포화선상피중양성세포최다、착색최심，차수임신시기추이축점증강；기층착색세포염색교천혹무，장막층궤호무착색。자궁조직중 GPR30상대표체량정임신전기＜임신중기＜임신후기적추세，임신조기표체량최저차겁현저저우임신중기화후기（P ＜0．01），임신후기표체량체최고치，단여임신중기무현저차별（P ＞0．05）。GPR30재임신기관중내산양자궁적분포화표체구유일정적규률차주요통과대자궁내막분비적조절삼여임신급분면등생리활동。
To investigate expression of G-protein coupled receptor 30 (GPR30)in uterus of goats during pregnancy.Immunochemistry SP method was used to detect the distribution of GPR30 and its protein a-bundance during the early pregnancy stage (1-30 d),mid-term pregnancy stage (31-120 d)and late preg-nancy stage (121-150 d).Different degree of GPR30 positive immunoreactions were observed in the endo-metrial layer,myometrium and serosa of uterus in dairy goats.GPR30 mainly located in the cell membrane and cytoplasm,especially in endometrial epithelial cells and glandular epithelium cells,while myometrium and serosa showed few or none immunoreactions.The staining was increased in endometrial epithelial cells and glandular epithelium with the progress of pregnancy.The relative level of GPR30 in uterus showed a trend of early pregnancy stage < mid-term pregnancy stage < late pregnancy stage,and the expression of GPR30 at the early stage of pregnancy was extremely lower than that of mid-term pregnancy stage and late pregnancy stage (P <0.01).GPR30 expression is maximal in the late pregnancy,but no significant differ-ence compared with mid-term pregnancy stage.GPR30 distribution and expression in the uterus of preg-nancy dairy goats showed certain regularity and mainly through the regulation of endometrial secretion in-volved in pregnancy and delivery and other physiological activities.
