动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2013年
12期
74-78,79
,共6页
鲍伟华%周雪荣%卢黎明%孙鹏烈%樊莉%方维焕%李肖梁
鮑偉華%週雪榮%盧黎明%孫鵬烈%樊莉%方維煥%李肖樑
포위화%주설영%로려명%손붕렬%번리%방유환%리초량
猪繁殖与呼吸综合征病毒%猪园环病毒2型Cap蛋白%卵黄免疫球蛋白%保育猪
豬繁殖與呼吸綜閤徵病毒%豬園環病毒2型Cap蛋白%卵黃免疫毬蛋白%保育豬
저번식여호흡종합정병독%저완배병독2형Cap단백%란황면역구단백%보육저
PRRSV%PCV-2-Cap protein%egg yolk immunoglobulin (IgY)%weaning piglets
应用特异性抗猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV-2)Cap 蛋白卵黄免疫球蛋白(IgY)饲喂保育猪,观察其对保育猪生长和免疫性能的影响。经抗体检测表明,53日龄时,PRRSV和 PCV-2抗体阴性率0.5%,试验组比对照组分别高15%和5%。在53日龄和60日龄时,保育猪 PRRSV阳性抗体 S/P 均值,试验组比对照组分别低15.59%和24.53%。保育猪46日龄~60日龄之间,阳性 PCV-2抗体 S/P 均值试验组比对照组分别低15.42%、19.66%和12.70%。保育猪转栏时,试验组比对照组平均末重高12.74%(P <0.01),平均日增重提高16.90%(P <0.01),成活率高1.67%。免疫组化结果显示,相对于空白组,添加组空肠和小肠组织中的 PRRSV 抗原的阳性表达几乎为0。试验结果为特异性抗体用于PRRSV 和 PCV-2的防控提供了参考。
應用特異性抗豬繁殖與呼吸綜閤徵病毒(PRRSV)和豬圓環病毒2型(PCV-2)Cap 蛋白卵黃免疫毬蛋白(IgY)飼餵保育豬,觀察其對保育豬生長和免疫性能的影響。經抗體檢測錶明,53日齡時,PRRSV和 PCV-2抗體陰性率0.5%,試驗組比對照組分彆高15%和5%。在53日齡和60日齡時,保育豬 PRRSV暘性抗體 S/P 均值,試驗組比對照組分彆低15.59%和24.53%。保育豬46日齡~60日齡之間,暘性 PCV-2抗體 S/P 均值試驗組比對照組分彆低15.42%、19.66%和12.70%。保育豬轉欄時,試驗組比對照組平均末重高12.74%(P <0.01),平均日增重提高16.90%(P <0.01),成活率高1.67%。免疫組化結果顯示,相對于空白組,添加組空腸和小腸組織中的 PRRSV 抗原的暘性錶達幾乎為0。試驗結果為特異性抗體用于PRRSV 和 PCV-2的防控提供瞭參攷。
응용특이성항저번식여호흡종합정병독(PRRSV)화저원배병독2형(PCV-2)Cap 단백란황면역구단백(IgY)사위보육저,관찰기대보육저생장화면역성능적영향。경항체검측표명,53일령시,PRRSV화 PCV-2항체음성솔0.5%,시험조비대조조분별고15%화5%。재53일령화60일령시,보육저 PRRSV양성항체 S/P 균치,시험조비대조조분별저15.59%화24.53%。보육저46일령~60일령지간,양성 PCV-2항체 S/P 균치시험조비대조조분별저15.42%、19.66%화12.70%。보육저전란시,시험조비대조조평균말중고12.74%(P <0.01),평균일증중제고16.90%(P <0.01),성활솔고1.67%。면역조화결과현시,상대우공백조,첨가조공장화소장조직중적 PRRSV 항원적양성표체궤호위0。시험결과위특이성항체용우PRRSV 화 PCV-2적방공제공료삼고。
Weaning piglets were fed with specific egg yolk immunoglobulin (IgY)antibodies against porcine reproductive and respiratory syndrome virus (PRRSV)and porcine circovirus type 2(PCV-2)-Cap protein, of which on growth performance and immunity was investigated.The results indicated that PRRSV and PCV-2 antibody negative rate was higher 15% and 5% in 0.5% groups than that of in blank groups at 53 days of age,respectively.Compared with blank groups,S/P average value of positive PRRSV antibody ti-ter in 0.5% groups decreased 15.59% and 24.53% over 53 and 60 days of age,respectively.During 46 to 60 days of age,SP average value of postive PCV-2 antibody titer of 0.5% groups were lower 15.42%, 19.66%and 12.70% than that of in blank groups,respectively.Compared with blank groups,finial body weight,daily weight gain and survival rate of 0.5% groups were improved 12.74% (P < 0.01),16.90%(P < 0.01)and 1.67% at weaning piglets,respectively.The expressions of PRRSV antigen in 0.5%groups were abolished in jejunum and small intestine with immunohistochemistry analysing,compared with the blank groups.The special IgY additive could provide a novel alternative approach to prevent and control diseases caused by PRRSV and PCV-2 infection in pigs.