CAJ | 학술논문

为了解2007年以来重庆地区猪圆环病毒2型（PCV-2）的流行情况，对2007年-2013年重庆地区送检样品进行了 PCV-2荧光定量 PCR 检测，并对阳性样品进行了病毒的分离鉴定，成功分离到12株PCV-2，对分离的12株 PCV-2进行了全基因组克隆与序列分析。结果显示，所分离的 PCV-2均处于 PCV-2b 分支，基因组全长均为1767 bp，与 PCV-2b 参考毒株（AF055394）的核苷酸同源性为96．2％～99．7％，与疫苗 SH 株的核苷酸同源性为96．0％～98．4％，而与疫苗 LG 株同源性仅为95．5％～95．8％。研究结果为 PCV-2的研究及其感染的防控奠定了基础，也为重庆地区 PCV-2疫苗毒株的选择提供了理论依据。
위료해2007년이래중경지구저원배병독2형（PCV-2）적류행정황，대2007년-2013년중경지구송검양품진행료 PCV-2형광정량 PCR 검측，병대양성양품진행료병독적분리감정，성공분리도12주PCV-2，대분리적12주 PCV-2진행료전기인조극륭여서렬분석。결과현시，소분리적 PCV-2균처우 PCV-2b 분지，기인조전장균위1767 bp，여 PCV-2b 삼고독주（AF055394）적핵감산동원성위96．2％～99．7％，여역묘 SH 주적핵감산동원성위96．0％～98．4％，이여역묘 LG 주동원성부위95．5％～95．8％。연구결과위 PCV-2적연구급기감염적방공전정료기출，야위중경지구 PCV-2역묘독주적선택제공료이론의거。
To learn about the prevalence of porcine circovirus type 2(PCV-2)in Chongqing,swine samples collected from 2007 to 2013 were detected by real-time PCR,and 12 local PCV-2 strains were identified and isolated.Sequence analysis shows that all the strains belongs to PCV-2b,and all the full length of PCV-2 genome is 1 767 bp,the homology of these trains is 96.2%-99.7% with classical strain AF055394 ,which is 96.0%-98.4% with SH strain and 95.5%-95.8% with LG strain respectively.This study got the basic epidemiological data for the prevention and control of PCV-2 in Chongqing area,it can also help the choice of the right PCV-2 vaccine.