动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2013年
12期
51-54
,共4页
熊炜%蒋静%张强%盘宝进%李健%魏晓锋%黄忠荣%胡建华
熊煒%蔣靜%張彊%盤寶進%李健%魏曉鋒%黃忠榮%鬍建華
웅위%장정%장강%반보진%리건%위효봉%황충영%호건화
猴%猴逆转录病毒%RT-PCR%real-time RT-PCR%Taq Man 探针
猴%猴逆轉錄病毒%RT-PCR%real-time RT-PCR%Taq Man 探針
후%후역전록병독%RT-PCR%real-time RT-PCR%Taq Man 탐침
monkey%Simian type D retrovirus%RT-PCR%real-time RT-PCR%Taq Man probe
猴逆转录病毒（Simian type D retrovirus，SRV）是引起猴获得性免疫缺陷综合征（Simian ac-quired immunodeficiency syndrome，SAIDS）的病原之一，其严重危害猴的健康，并威胁与猴接触人员的健康，是无特定病原体（SPF）猴必须排除的病毒之一。为了应对口岸对进出境野生及实验用灵长类动物 SRV感染情况的监测和流行病学调查的需要，建立了 RT-PCR 和 real-time RT-PCR 检测 SRV 的方法，并对方法的特异性、敏感性和稳定性进行了验证。
猴逆轉錄病毒（Simian type D retrovirus，SRV）是引起猴穫得性免疫缺陷綜閤徵（Simian ac-quired immunodeficiency syndrome，SAIDS）的病原之一，其嚴重危害猴的健康，併威脅與猴接觸人員的健康，是無特定病原體（SPF）猴必鬚排除的病毒之一。為瞭應對口岸對進齣境野生及實驗用靈長類動物 SRV感染情況的鑑測和流行病學調查的需要，建立瞭 RT-PCR 和 real-time RT-PCR 檢測 SRV 的方法，併對方法的特異性、敏感性和穩定性進行瞭驗證。
후역전록병독（Simian type D retrovirus，SRV）시인기후획득성면역결함종합정（Simian ac-quired immunodeficiency syndrome，SAIDS）적병원지일，기엄중위해후적건강，병위협여후접촉인원적건강，시무특정병원체（SPF）후필수배제적병독지일。위료응대구안대진출경야생급실험용령장류동물 SRV감염정황적감측화류행병학조사적수요，건립료 RT-PCR 화 real-time RT-PCR 검측 SRV 적방법，병대방법적특이성、민감성화은정성진행료험증。
Simian type D retrovirus(SRV)is one of pathogens led to simian acquired immunodeficiency syndrome (SAIDS),which is harmful for health of monkey and its engaged person.And it is also one of virus must be elimi-nated from specefic pathogen free (SPF)monkey.In order to survey and investigate the epidemic character of SRV transmission among wild and experimental monkeys,methods of RT-PCR and real-time RT-PCR using Taq Man probe to detect SRV were established and proved to be specific with high sensitivity and stability.
