CAJ | 학술논문

从广西32家养殖场分别按100头以上母猪和100头以下母猪的不同规模猪场采集经过猪瘟疫苗一免、二免和三免后1个月的血清1083份，使用阻断 ELISA 方法对样品的猪瘟抗体水平进行检测。结果表明，一免和二免抗体合格率相比差异不显著，但和三免相比差异显著。不同免疫次数的抗体离散度差异不显著，100头母猪以上的猪场抗体离散度低于100头母猪以下的猪场。结果显示猪瘟抗体三免后效果最好，100头以下的猪场应加强对猪群猪瘟抗体的监测，根据个体的抗体消长规律进行有针对性的免疫，以提高群体抗体均匀度。
종엄서32가양식장분별안100두이상모저화100두이하모저적불동규모저장채집경과저온역묘일면、이면화삼면후1개월적혈청1083빈，사용조단 ELISA 방법대양품적저온항체수평진행검측。결과표명，일면화이면항체합격솔상비차이불현저，단화삼면상비차이현저。불동면역차수적항체리산도차이불현저，100두모저이상적저장항체리산도저우100두모저이하적저장。결과현시저온항체삼면후효과최호，100두이하적저장응가강대저군저온항체적감측，근거개체적항체소장규률진행유침대성적면역，이제고군체항체균균도。
1 083 pig serum samples were collected from 32 farms,16 of these farms had more than 100 sows and the other 16 had less than 100 sows.These serum samples were collected on 1 month after prime,first boost and second boost vaccinations with CSF vaccine,respectively.The antibody levels of these serum samples were measured by blocking-ELISA technique.The result showed that there is no significant difference in CSFV antibodies between the serums from the prime immunization and the first boost immu-nization,but the difference is significant compared with the second boost immunization.The antibody dis-persion is obviously high in pig sera from farms less than 100 sows,while there is no significant difference in antibody dispersion after different immunizations.The result indicated that those pigs received the third immunization had optimal antibody levels.To reduce the antibody dispersion,those farms less than 100 sows should establish and optimize the vaccination procedure basing on the results of antibody surveillance in different herds of pigs.