医学理论与实践
醫學理論與實踐
의학이론여실천
The Journal of Medical Theory and Practice
2013年
24期
3244-3246,3252
,共4页
朱凤丹%李多孚%张健%尹秀杉
硃鳳丹%李多孚%張健%尹秀杉
주봉단%리다부%장건%윤수삼
血培养%病原菌%耐药率
血培養%病原菌%耐藥率
혈배양%병원균%내약솔
Blood culture%Pathogenic bacteria%Drug resistance rates
目的：分析我院2012年1月-2013年7月血培养病原菌分布及耐药性。方法：用BACTEC9120血培养仪，细菌及真菌鉴定使用A PI系统及M icroScan鉴定系统，药物敏感试验用纸片扩散法（KB法）及肉汤稀释法，药敏结果判断和解释参照抗菌药物敏感试验执行标准（CLSI 2010版）[1]，采用WHONET5．4软件统计分析。结果：7415份标本检出病原菌641株，阳性率为8．64％。2012年检出病原菌374株，其中革兰阳性球菌188株，革兰阴性杆菌162株，真菌24株。2013年检出病原菌267株，其中革兰阳性球菌134株，革兰阴性杆菌120株，真菌13株。金黄色葡萄球菌和表皮葡萄球菌对利奈唑烷、万古霉素、达托霉素敏感率为100％；大肠埃希菌和肺炎克雷伯菌对亚胺培南敏感率为100％。结论：2012年1月-2013年7月血培养病原菌检出病原菌种类多、分布广且耐药性较严重。但大部分抗菌药物耐药率2013年比2012年低（ P＜0．005），说明我院从2010年开始进行抗生素规范使用，已对控制细菌耐药产生重要影响，并初步取得成果。
目的：分析我院2012年1月-2013年7月血培養病原菌分佈及耐藥性。方法：用BACTEC9120血培養儀，細菌及真菌鑒定使用A PI繫統及M icroScan鑒定繫統，藥物敏感試驗用紙片擴散法（KB法）及肉湯稀釋法，藥敏結果判斷和解釋參照抗菌藥物敏感試驗執行標準（CLSI 2010版）[1]，採用WHONET5．4軟件統計分析。結果：7415份標本檢齣病原菌641株，暘性率為8．64％。2012年檢齣病原菌374株，其中革蘭暘性毬菌188株，革蘭陰性桿菌162株，真菌24株。2013年檢齣病原菌267株，其中革蘭暘性毬菌134株，革蘭陰性桿菌120株，真菌13株。金黃色葡萄毬菌和錶皮葡萄毬菌對利奈唑烷、萬古黴素、達託黴素敏感率為100％；大腸埃希菌和肺炎剋雷伯菌對亞胺培南敏感率為100％。結論：2012年1月-2013年7月血培養病原菌檢齣病原菌種類多、分佈廣且耐藥性較嚴重。但大部分抗菌藥物耐藥率2013年比2012年低（ P＜0．005），說明我院從2010年開始進行抗生素規範使用，已對控製細菌耐藥產生重要影響，併初步取得成果。
목적：분석아원2012년1월-2013년7월혈배양병원균분포급내약성。방법：용BACTEC9120혈배양의，세균급진균감정사용A PI계통급M icroScan감정계통，약물민감시험용지편확산법（KB법）급육탕희석법，약민결과판단화해석삼조항균약물민감시험집행표준（CLSI 2010판）[1]，채용WHONET5．4연건통계분석。결과：7415빈표본검출병원균641주，양성솔위8．64％。2012년검출병원균374주，기중혁란양성구균188주，혁란음성간균162주，진균24주。2013년검출병원균267주，기중혁란양성구균134주，혁란음성간균120주，진균13주。금황색포도구균화표피포도구균대리내서완、만고매소、체탁매소민감솔위100％；대장애희균화폐염극뢰백균대아알배남민감솔위100％。결론：2012년1월-2013년7월혈배양병원균검출병원균충류다、분포엄차내약성교엄중。단대부분항균약물내약솔2013년비2012년저（ P＜0．005），설명아원종2010년개시진행항생소규범사용，이대공제세균내약산생중요영향，병초보취득성과。
Objective :To analysis the distribution and drug resistance of pathogenic bacteria in blood culture in central hospital of Nanchong from 2012 to July 2013 .Methods :Blood samples were cultivated with BACTEC9120 Blood culture system ,bacteria and fungi were identified with API system and MicroScan identification system ,drug sensitivity test was taken with KB method and Broth dilution method .Judgment and interpretation were reference to the implementa-tion of standards (CLSI 2010) ,the test results were analyzed with WHONET 5 .4 software .Results:Among the 7 415 clinical blood samples ,641 strains of pathogenic bacteria were isolated ,the positive rate was 8 .64% .374 strains of pathogenic bacteria were isolated in 2012 ,Gram-positive cocci were 188 strains ,Gram-negative bacilli were 162 strains , fungi were 24 stains .267 strains of pathogenic bacteria were isolated in 2013 ,Gram-positive cocci were 134 strains , Gram-negative bacilli were 120 strains ,fungi were 13 stains .Staphylococcus aureus and Staphylococcus epidermidis were sensitive (100% ) to Linezolid ,Vancomycin and Daptomycin .Escherichia coli and Klebsiella pneumoniae were sensitive (100% ) to Imipenem .Conclusion:From January 2012 to July 2013 blood culture pathogens were detected Spe-cies ,widely distributed and more serious resistance .But most antimicrobial resistance rates were lower in 2013 than 2012(P<0 .005) ,the explanation was that our hospital began to regulate the use of antibiotic from 2010 ,and it has im-portant impact on the control of bacterial resistance ,and preliminary results achieved .
