仲恺农业工程学院学报
仲愷農業工程學院學報
중개농업공정학원학보
JOURNAL OF ZHONGKAI UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
2013年
4期
1-7
,共7页
叶婵娟%刘明锋%刘文%周玲艳%杨妙贤%胡延吉%梁红
葉嬋娟%劉明鋒%劉文%週玲豔%楊妙賢%鬍延吉%樑紅
협선연%류명봉%류문%주령염%양묘현%호연길%량홍
猕猴桃 ( Actinidia spp.)%DNA条形码%rbcL基因%UPGMA聚类%多样性分析
獼猴桃 ( Actinidia spp.)%DNA條形碼%rbcL基因%UPGMA聚類%多樣性分析
미후도 ( Actinidia spp.)%DNA조형마%rbcL기인%UPGMA취류%다양성분석
kiwifruit (Actinidia spp.)%DNA Barcoding%rbcL gene%UPGMA clustering%diversity anal-ysis
利用clustal W软件对42个猕猴桃（ Actinidia spp．）品种及种内不同性别间的rbcL基因序列进行了亲缘关系分析，还根据“和平红阳”猕猴桃雌株和雄株的叶绿体基因rbcL序列与GenBank的其他猕猴桃rbcL基因序列进行UPGMA聚类比对．结果表明，广东省野生猕猴桃种质资源中rbcL基因序列具有一定的多样性，猕猴桃野生种rbcL基因的多样性高于栽培品种和种内不同性别．通过对猕猴桃clustal W亲缘关系分析和UPGMA聚类比对，显示猕猴桃种间的rbcL基因差异性明显，可分为4组类群．认为rbcL基因的DNA条形码技术适用于猕猴桃属植物的多样性研究，将为猕猴挑的系统发育研究和猕猴桃资源保育提供一种研究思路和技术方法．
利用clustal W軟件對42箇獼猴桃（ Actinidia spp．）品種及種內不同性彆間的rbcL基因序列進行瞭親緣關繫分析，還根據“和平紅暘”獼猴桃雌株和雄株的葉綠體基因rbcL序列與GenBank的其他獼猴桃rbcL基因序列進行UPGMA聚類比對．結果錶明，廣東省野生獼猴桃種質資源中rbcL基因序列具有一定的多樣性，獼猴桃野生種rbcL基因的多樣性高于栽培品種和種內不同性彆．通過對獼猴桃clustal W親緣關繫分析和UPGMA聚類比對，顯示獼猴桃種間的rbcL基因差異性明顯，可分為4組類群．認為rbcL基因的DNA條形碼技術適用于獼猴桃屬植物的多樣性研究，將為獼猴挑的繫統髮育研究和獼猴桃資源保育提供一種研究思路和技術方法．
이용clustal W연건대42개미후도（ Actinidia spp．）품충급충내불동성별간적rbcL기인서렬진행료친연관계분석，환근거“화평홍양”미후도자주화웅주적협록체기인rbcL서렬여GenBank적기타미후도rbcL기인서렬진행UPGMA취류비대．결과표명，광동성야생미후도충질자원중rbcL기인서렬구유일정적다양성，미후도야생충rbcL기인적다양성고우재배품충화충내불동성별．통과대미후도clustal W친연관계분석화UPGMA취류비대，현시미후도충간적rbcL기인차이성명현，가분위4조류군．인위rbcL기인적DNA조형마기술괄용우미후도속식물적다양성연구，장위미후도적계통발육연구화미후도자원보육제공일충연구사로화기술방법．
Using clustal W software to analysis the genetic relationship between the rbcL sequences in 42 Actinidia spp.and within different gender .The determined rbcL sequence of Actinidia chinensis cv He-ping Hongyang , and the highly similar sequences in the GenBank DNA database were aligned , then the MEGA4.0 software was used to construct a dendrogram by UPGMA clustering analysis .The results showed that the wild kiwifruit germplasm resources in Guangdong province are of diversity .The diversity of rbcL gene from wild kiwifruit is higher than that of cultivars and species in different gender .The UPG-MA clustering revealed that the diversity is obvious in rbcL gene of kiwifruit species and kiwifruits can be divided into 4 groups of taxa .Based on the study on the genetic relationship analysis by clustal W and UPGMA clustering algorithm , rbcL gene is applicable to being DNA barcoding for diversity analysis .It will provide a new train of thoughts and methods to study in phylogeny and resource conservation of ki -wifruit.
