CAJ | 학술논문

利用兰州重离子研究装置(HIRFL)提供的12C6+离子束辐照菘蓝干种子(辐照剂量为10，35，60，90和140 Gy，剂量率20 Gy/min)，探讨了重离子束辐照对菘蓝M1代的生物学效应。研究发现，不同剂量的12C6+离子束辐照后，菘蓝种子的发芽率、成苗率、株高、根长和根冠比等生物学性状以及对菘蓝中靛玉红和4(3H)喹唑酮含量均发生了变化，其中株高和根长随辐照剂量的增加而降低；菘蓝叶和根中的4(3H)喹唑酮和靛玉红的含量随辐照剂量增加呈马鞍形增加关系。这表明：12C6+重离子束辐照菘蓝种子具有明显的当代损伤效应,并可显著提高菘蓝中靛玉红和4(3H)喹唑酮的含量，其辐照适宜诱变剂量为35 Gy。
이용란주중리자연구장치(HIRFL)제공적12C6+리자속복조숭람간충자(복조제량위10，35，60，90화140 Gy，제량솔20 Gy/min)，탐토료중리자속복조대숭람M1대적생물학효응。연구발현，불동제량적12C6+리자속복조후，숭람충자적발아솔、성묘솔、주고、근장화근관비등생물학성상이급대숭람중전옥홍화4(3H)규서동함량균발생료변화，기중주고화근장수복조제량적증가이강저；숭람협화근중적4(3H)규서동화전옥홍적함량수복조제량증가정마안형증가관계。저표명：12C6+중리자속복조숭람충자구유명현적당대손상효응,병가현저제고숭람중전옥홍화4(3H)규서동적함량，기복조괄의유변제량위35 Gy。
To investigate the M1 biological effects of heavy ions on Isatis indigotica Fort, its dry seeds were irradiated by 12C6+ beam with the dose of 0, 10, 35, 60, 90 and 140 Gy respectively,at the rate of 20 Gy/min delivered by the Heavy Ion Research Facility in Lanzhou (HIRFL). The results showed that biological characters such as germinating rate, germinating potential, survival rate, plant height, root height and root-shoot ratio were changed after irradiation. Moreover, the plant height and root height decreased in a dos dependent manner. The indirubin and 4(3H) quinazolinone content of Isatis indigotica Fort was improved and exhibited obviously“saddle”trends with irradiation dose increasing. Data suggest that exposure with low-dose 12C6+ to seeds of Isatis indigotica Fort has obvious injury effects at the first generation, and the active ingredient content of Isatis indigotica Fort may be improved by carbon ion beam irradiation. It is concluded that the suitable irradiation dose of mutation breeding is 35 Gy for the seeds of Isatis indigotica Fort.