CAJ | 학술논문

体外研究再生丝素蛋白材料对小鼠骨髓来源树突状细胞分化、成熟的影响，探讨丝素蛋白的免疫原性及作为生物材料的意义。体外分离培养小鼠骨髓来源树突状细胞，接种于铺有再生丝素蛋白生物材料的细胞培养板中培养，在光学显微镜下观察细胞形态；FCM分析细胞表面相关分子的表达。同时用 MTT 法测小鼠骨髓来源树突状细胞细胞株 DC2．4在所选各种材料表面的生长增殖情况。结果表明：（1）再生柞蚕丝素蛋白和家蚕丝素蛋白均可支持小鼠骨髓来源的 DCs 生长和聚集；（2）MTT 结果表明再生柞蚕丝素蛋白与再生家蚕丝素蛋白相比可促进小鼠 BM来源 DC2．4的增殖；（3）再生柞蚕丝素蛋白和再生家蚕丝素蛋白上的小鼠 BM来源 DCs 表面低表达 CD11c、CD40、CD80、CD86、MHC -Ⅱ分子，与空白对照组 DCs 的表达谱相比无明显差别。再生柞蚕丝素蛋白与再生家蚕丝素蛋白相比，可较好地支持小鼠 BM来源 DC2．4的生长和增殖；再生丝素蛋白无刺激小鼠 BM来源 DCs 成熟的生物学效应。
체외연구재생사소단백재료대소서골수래원수돌상세포분화、성숙적영향，탐토사소단백적면역원성급작위생물재료적의의。체외분리배양소서골수래원수돌상세포，접충우포유재생사소단백생물재료적세포배양판중배양，재광학현미경하관찰세포형태；FCM분석세포표면상관분자적표체。동시용 MTT 법측소서골수래원수돌상세포세포주 DC2．4재소선각충재료표면적생장증식정황。결과표명：（1）재생작잠사소단백화가잠사소단백균가지지소서골수래원적 DCs 생장화취집；（2）MTT 결과표명재생작잠사소단백여재생가잠사소단백상비가촉진소서 BM래원 DC2．4적증식；（3）재생작잠사소단백화재생가잠사소단백상적소서 BM래원 DCs 표면저표체 CD11c、CD40、CD80、CD86、MHC -Ⅱ분자，여공백대조조 DCs 적표체보상비무명현차별。재생작잠사소단백여재생가잠사소단백상비，가교호지지지소서 BM래원 DC2．4적생장화증식；재생사소단백무자격소서 BM래원 DCs 성숙적생물학효응。
To investigate the effect of regenerated silk fibroin on the differentiation and maturation of murine BM-derived DCs in vitro.In order to clarify the immunogenicity and biocompatibility of silk fibroin.Murine BM-derived DCs were Isolated and cultured in vitro and then transferred to the wells coated with silk fibroin.The cell morphology was observed with microscope.And the proliferation of the cells from each group was determined by MTT assay.The expressions of associated molecules were analyzed with flow cytometry. Results showed that (1)Both regenerated Antheraea pernyi silk fibroin and regenerated Bombyx mori silk fibroin could support the grow and aggregate formation of murine DCs;(2)MTT assay showed that regenerated Antheraea pernyi silk fibroin could support the proliferation of DC2.4 significantly;(3)By FCM analyses,the expression pattern of CD11c,CD40,CD80,CD86 and MHC -Ⅱmolecules on BM-derived DCs were similar between silk fibron group and blank group.Regenerated Antheraea pernyi silk fibroin can support the growth and proliferation of DC2.4 better than regenerated Bombyx mori silk fibroin did.Regenerated silk fibroin can not promote the maturation of murine BM-derived DCs.