CAJ | 학술논문

目的：探讨独角莲醇提液对H22肝癌荷瘤小鼠血清IL-2和TNF-α水平的影响。方法建立体内H22肝癌荷瘤小鼠的模型，采用灌胃法给予其100mg·kg-1·d-1独角莲醇提液，以生理盐水灌胃和5-FU腹腔注射作为对照，连续10d，停药后第2天摘取小鼠眼球取血后断髓处死，取出胸腺、脾脏称重，计算小鼠的胸腺重量指数、脾脏重量指数，采用ELISA法检测小鼠血清中细胞因子白介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）的水平。结果与生理盐水灌胃的H22肝癌荷瘤小鼠比较，5-FU腹腔注射和独角莲醇提液灌胃处理的H22荷瘤鼠血清中细胞因子IL-2、TNF-α水平明显升高；但胸腺重量指数、脾脏重量指数显著下降，差异均具有统计学意义（P<0.05）。结论独角莲醇提液可提高H22荷瘤鼠的血清IL-2和TNF-α水平。
목적：탐토독각련순제액대H22간암하류소서혈청IL-2화TNF-α수평적영향。방법건입체내H22간암하류소서적모형，채용관위법급여기100mg·kg-1·d-1독각련순제액，이생리염수관위화5-FU복강주사작위대조，련속10d，정약후제2천적취소서안구취혈후단수처사，취출흉선、비장칭중，계산소서적흉선중량지수、비장중량지수，채용ELISA법검측소서혈청중세포인자백개소-2（IL-2）、종류배사인자-α（TNF-α）적수평。결과여생리염수관위적H22간암하류소서비교，5-FU복강주사화독각련순제액관위처리적H22하류서혈청중세포인자IL-2、TNF-α수평명현승고；단흉선중량지수、비장중량지수현저하강，차이균구유통계학의의（P<0.05）。결론독각련순제액가제고H22하류서적혈청IL-2화TNF-α수평。
Objective To explore the effects of alcohol extracts of Typhonium giganteum Engl. on the serum IL-2 and TNF-alpha levels of H22 liver cancer-bearing mice. Methods The models of in vivo H22 cancer bearing mice were established, and 10 mice were lavaged with 100 mg·kg-1·d-1 of alcohol extracts of Typhonium giganteum Engl., and another 10 had intra-peritoneal injection of 5-FU, and the remaining 10 had lavage of saline and were taken as control, for 10 days. On the 2nd day after drug discontinuation, the mice were pulpotomy executed after being taken blood from eyes, and then the thymus, spleen were removed, weighed and calculated. The serum interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-α) levels were detected by ELISA. Results Compared with the mice lavaged by saline, the serum IL-2 and TNF-αlevels of mice with intra-peritoneal injection of 5-FU and those with Typhonium giganteum Engl. alcohol extracts significantly increased, but their thymus weight index, spleen weight index both decreased (P<0.05). Differences all had statistical significance. Conclusion The alcohol extracts of Typhonium giganteum Engl. could enhance serum IL-2 and TNF-αlevels of H22 liver cancer-bearing mice.