CAJ | 학술논문

利用RAPD技术分别对分离自黑龙江省各地区的90株链格孢属病原菌进行了遗传多样性分析。利用NTSYS 2.0进行链格孢属病原菌的聚类分析。RAPD分析结果表明，引物OPE-19扩增后产生的各个群体间相似性系数分布在0.60~1.00。在68.0%相似性水平上可划分为3个主要RAPD组群和8个次要RAPD组群。聚类分析结果表明，黑龙江省链格孢属病原菌RAPD组群与寄主植物和分离地区之间没有相关性。
이용RAPD기술분별대분리자흑룡강성각지구적90주련격포속병원균진행료유전다양성분석。이용NTSYS 2.0진행련격포속병원균적취류분석。RAPD분석결과표명，인물OPE-19확증후산생적각개군체간상사성계수분포재0.60~1.00。재68.0%상사성수평상가화분위3개주요RAPD조군화8개차요RAPD조군。취류분석결과표명，흑룡강성련격포속병원균RAPD조군여기주식물화분리지구지간몰유상관성。
To understand the genetic diversity of 90 Alternaria pathogen isolates col ected from Heilongjiang Province, the genotypic characteristics was performed by using random amplified polymorphic DNA (RAPD), and cluster analysis of Alternaria pathogens was carried out by using NTSYs program. RAPD results indicated that the similarity coefficients of RAPD group amplified by the primer OPE-19 ranged from 0.60 to 1.00. According to RAPD analysis by using the primer OPE-19, 90 isolates were separated into three major RAPD groups and eight minor groups at the similarity level of 68.0%. The cluster analysis results suggested that RAPD groups not be correlated with the host plants and locations where the isolates were col ected.