CAJ | 학술논문

马铃薯纺锤块茎类病毒（PSTVd）是威胁我国马铃薯生产的重要病害之一，目前有多种技术可应用于PSTVd的检测，如生物学方法、电子显微镜技术、往返聚丙烯酰胺凝胶电泳（R-PAGE）、核酸斑点杂交（NASH）、RT-PCR和FQ RT-PCR。这些方法各有利弊，互相补充，构成了PSTVd的检测技术体系。日常工作中，应根据自身条件及目的等科学地选择适宜的检测技术，确保脱毒种薯/种苗的质量。
마령서방추괴경류병독（PSTVd）시위협아국마령서생산적중요병해지일，목전유다충기술가응용우PSTVd적검측，여생물학방법、전자현미경기술、왕반취병희선알응효전영（R-PAGE）、핵산반점잡교（NASH）、RT-PCR화FQ RT-PCR。저사방법각유리폐，호상보충，구성료PSTVd적검측기술체계。일상공작중，응근거자신조건급목적등과학지선택괄의적검측기술，학보탈독충서/충묘적질량。
Potato spindle tuber viroid (PSTVd) is one of the important diseases that threatens the potato production in China. Now, there are some techniques for detection of PSTVd, such as biological method, electron microscope technique, R-PAGE, NASH, RT-PCR and FQ RT-PCR. These methods have advantages and disadvantages and they can complement each other, comprising a system for PSTVd detection. Daily work should be based on their own conditions and purposes, and the detection method be appropriately chosen to control the virus-free seed/seedling quality.