CAJ | 학술논문

背景：有报道指出，孤独症谱系障碍(ASD)患儿的胃肠道(GI)症状发生率较高。然而，有关ASD患儿胃肠道微生物的研究结果不尽一致。 　　目的：系统复习相关研究结果，分析ASD患儿各种胃肠道微生物的分布特征。 　　方法：检索PubMed、Embase、PsycINFO、ISIwebofknowledge、Ovid/Medline、CochraneLibrary、中国知识资源总库、中国科技期刊数据库、万方数据检索系统，以及中国生物医学文摘数据库，收集有关ASD患者胃肠道微生物的文献，按照预先制定的纳入及排除标准筛选相关研究。采用Reviewmanger5.2.6软件进行统计分析。 　　结果：最终共纳入15项小样本横断面研究，其中1项来自于中国。在15项研究中，11项研究（合并样本为562例）报道ASD患儿组与对照组的胃肠道细菌患病率有显著性差异，尤其是厚壁菌，类杆菌和变形菌。但是，由于方法学上较大的异质性以及不同研究结果之间的相互矛盾，我们无法汇集结果进行meta分析。 　　结论：目前对ASD患儿胃肠道微生物的研究数量和质量都非常有限。然而，的确有一个“信号”表明ASD患儿的胃肠道微生物和没有ASD的儿童是有显著差异的，因此，继续开展此方面的研究是非常有价值的。为了提高研究的效度，减少研究结果的异质性，将来的研究需要增大样本，标准化方法以及评估相关混杂因素，例如胃肠道症状的严重程度，以及药物，特殊饮食和添加剂的使用情况。
배경：유보도지출，고독증보계장애(ASD)환인적위장도(GI)증상발생솔교고。연이，유관ASD환인위장도미생물적연구결과불진일치。 　　목적：계통복습상관연구결과，분석ASD환인각충위장도미생물적분포특정。 　　방법：검색PubMed、Embase、PsycINFO、ISIwebofknowledge、Ovid/Medline、CochraneLibrary、중국지식자원총고、중국과기기간수거고、만방수거검색계통，이급중국생물의학문적수거고，수집유관ASD환자위장도미생물적문헌，안조예선제정적납입급배제표준사선상관연구。채용Reviewmanger5.2.6연건진행통계분석。 　　결과：최종공납입15항소양본횡단면연구，기중1항래자우중국。재15항연구중，11항연구（합병양본위562례）보도ASD환인조여대조조적위장도세균환병솔유현저성차이，우기시후벽균，류간균화변형균。단시，유우방법학상교대적이질성이급불동연구결과지간적상호모순，아문무법회집결과진행meta분석。 　　결론：목전대ASD환인위장도미생물적연구수량화질량도비상유한。연이，적학유일개“신호”표명ASD환인적위장도미생물화몰유ASD적인동시유현저차이적，인차，계속개전차방면적연구시비상유개치적。위료제고연구적효도，감소연구결과적이질성，장래적연구수요증대양본，표준화방법이급평고상관혼잡인소，례여위장도증상적엄중정도，이급약물，특수음식화첨가제적사용정황。
Background:A high prevalence of gastrointestinal (GI) symptoms has been reported in children with Autism Spectrum Disorders (ASD). However, results from studies about the GI mircobiome of such children have been inconsistent. Aim:Integrate the results of studies that examine the distribution of different GI microorganisms in children with ASD. Methods:Studies related to the GI microbiome in children with ASD were identified through PubMed, Embase, PsycINFO, ISI web of knowledge, Ovid/Medline, the Cochrane Library, the Chinese National Knowledge Infrastructure (CNKI) database, the Chongqing VIP database for Chinese Technical Periodicals, WANFANG DATA, and the China BioMedical Literature Service System (SinoMed). Studies were screened for inclusion following pre-defined inclusion and exclusion criteria. Software Review Manager 5.2.6 was used for statistical analysis. Results:A total of 15 cross-sectional studies, all of which had relatively small samples, were included in the final analysis. Only one of the included studies was from China. Among the 15 studies, 11 studies (with a combined sample of 562 individuals) reported significant differences between ASD children and controls in the prevalence of GI bacteria, particularly bacteria in the Firmicutes, Bacteroidetes and Proteobacteria phyla. However, due to the substantial heterogeneity in methodology and the often contradictory results of different studies, it was not possible to pool the results into a meta-analysis. Conclusions:To date, studies on the GI microbiome in children with ASD are limited in quantity and quality. There does, however, appear to be a‘signal’ suggesting significant differences in the GI microbiome between ASD children and children without ASD, so there would be value in continuing this line of research. To improve validity and decrease the heterogeneity of findings, future studies should enlarge sample sizes, standardize methods and assess relevant confounding variables, such as the severity of GI symptoms and the use of medications, special diets and supplements.