CAJ | 학술논문

目的：研究一种便捷、高效地检测黄瓜花叶病毒（CMV）的方法。方法：利用表面等离子体共振（SPR）技术检测CMV。首先用11-MUA修饰SPR金片，再用EDC/NHS活化，之后通过NHS酯基与CMV抗体结合，用BSA封闭未结合的NHS酯基。将SPR金片装入SPR仪，通入待检样品，通过折射率变化实时监测实验过程。结果：该方法检测CMV的灵敏度能够达到10 ng/mL，具有良好的特异性，与同属的花生矮化病毒、番茄不孕病毒无交叉反应。结论：建立的SPR方法操作简单、灵敏度高、特异性好，是一种新的高效检测CMV的方法。
목적：연구일충편첩、고효지검측황과화협병독（CMV）적방법。방법：이용표면등리자체공진（SPR）기술검측CMV。수선용11-MUA수식SPR금편，재용EDC/NHS활화，지후통과NHS지기여CMV항체결합，용BSA봉폐미결합적NHS지기。장SPR금편장입SPR의，통입대검양품，통과절사솔변화실시감측실험과정。결과：해방법검측CMV적령민도능구체도10 ng/mL，구유량호적특이성，여동속적화생왜화병독、번가불잉병독무교차반응。결론：건립적SPR방법조작간단、령민도고、특이성호，시일충신적고효검측CMV적방법。
Objective: To research into a convenient and efficient detection methods of cucumber mosaic virus (CMV). Methods: Detection of CMV using surface plasmon resonance(SPR). First, we used 11-MUA to modify SPR gold crystal, then used EDC/NHS to active 11-MUA, followed by antibody binding to NHS esters. The un-bound NHS esters were blocked with BSA. Put SPR gold crystal into the SPR instrument, then inject into sample to be tested, we can monitor the experiment in real-time by the refractive index changes. Results: The detection limit of this method was 10 ng/mL. Also it had good specificity which had no cross reaction with peanut stunt vi-rus and tomato aspermy virus. Conclusion: The experimental results showed that this SPR method was simple op-eration and had high sensitivity and good specificity. We established a new efficient method for detection of CMV.