CAJ | 학술논문

目的：太岁是自然界中一种组成及分类尚不明确的生物体。采用分子生物学方法对2种不同来源地的太岁样品进行菌种分离培养和分子鉴定。方法：太岁表面经75%乙醇消毒处理后，用麦芽汁培养基分离可培养菌株，提取太岁及分离菌株基因组DNA，以之为模板，用16S rDNA、18S rDNA和ITS通用引物进行PCR扩增，扩增片段连入pMD18T载体并测序，通过序列比对对太岁菌种组成进行初步鉴定。结果：从太岁1号样品中分离到假丝酵母（Can-dida）和粘质红酵母（Rhodotorula mucilaginosa），从太岁2号样品中分离到根癌农杆菌（Agrobacterium tumefaciens）和粘质红酵母。结论：2种来源不同的太岁样品中均存在可培养的粘质红酵母。
목적：태세시자연계중일충조성급분류상불명학적생물체。채용분자생물학방법대2충불동래원지적태세양품진행균충분리배양화분자감정。방법：태세표면경75%을순소독처리후，용맥아즙배양기분리가배양균주，제취태세급분리균주기인조DNA，이지위모판，용16S rDNA、18S rDNA화ITS통용인물진행PCR확증，확증편단련입pMD18T재체병측서，통과서렬비대대태세균충조성진행초보감정。결과：종태세1호양품중분리도가사효모（Can-dida）화점질홍효모（Rhodotorula mucilaginosa），종태세2호양품중분리도근암농간균（Agrobacterium tumefaciens）화점질홍효모。결론：2충래원불동적태세양품중균존재가배양적점질홍효모。
Objective: Taisui is an organism with undefined classification existed in nature. The object of this study was to isolate and molecular identity strains in two Taisui samples obtained from different source by molecu- lar method. Methods: After washing with 75% ethanol for about 10 s, the Taisui samples were inoculated into malt wort medium to obtain the cultured strains. Genomic DNA of Taisui samples were isolated and used as the template to amplify 16S rDNA, 18S rDNA and ITS by PCR. The PCR fragments were then ligated into pMD18T and sequenced respectively. Strain identification was performed by sequence alignment against NCBI GenBank. Re- sults: Candida and Rhodotorula mucilaginosa were found in No.1 sample while Agrobacterium tumefaciens and R. mucilaginosa were found in No.2 sample. Conclusion: Rhodotorula mucilaginosa was found in these two Taisui samples from different source.