生物技术通讯
生物技術通訊
생물기술통신
LETTERS IN BIOTECHNOLOGY
2013年
6期
792-796
,共5页
傅春江%贾雅丽%周军年%陈琳%曾泉%南雪%岳文%尉承泽%裴雪涛
傅春江%賈雅麗%週軍年%陳琳%曾泉%南雪%嶽文%尉承澤%裴雪濤
부춘강%가아려%주군년%진림%증천%남설%악문%위승택%배설도
表皮形态发生素%肝细胞癌%增殖%浸润
錶皮形態髮生素%肝細胞癌%增殖%浸潤
표피형태발생소%간세포암%증식%침윤
epimorphin%hepatocellular carcinoma%proliferation%invasion
目的：明确表皮形态发生素（EPM）对肝细胞癌SK-HEP-1细胞生物学行为的影响。方法：构建高表达EPM的SK-HEP-1细胞，real-time PCR和Western印迹检测EPM在肿瘤细胞内的表达，CCK8分析和克隆形成实验检测细胞的增殖能力，Matrigel-transwell实验检测细胞的浸润能力。结果：EPM在肿瘤细胞内的高表达不影响细胞的增殖能力，但明显增强肿瘤细胞的浸润能力。结论：肝癌肿瘤微环境有可能通过EPM影响肿瘤细胞的生物学活性，对其作用机制的进一步明确，将有助于阐明肝癌发生发展的病理机制，发现新的恶性肿瘤诊断和治疗手段。
目的：明確錶皮形態髮生素（EPM）對肝細胞癌SK-HEP-1細胞生物學行為的影響。方法：構建高錶達EPM的SK-HEP-1細胞，real-time PCR和Western印跡檢測EPM在腫瘤細胞內的錶達，CCK8分析和剋隆形成實驗檢測細胞的增殖能力，Matrigel-transwell實驗檢測細胞的浸潤能力。結果：EPM在腫瘤細胞內的高錶達不影響細胞的增殖能力，但明顯增彊腫瘤細胞的浸潤能力。結論：肝癌腫瘤微環境有可能通過EPM影響腫瘤細胞的生物學活性，對其作用機製的進一步明確，將有助于闡明肝癌髮生髮展的病理機製，髮現新的噁性腫瘤診斷和治療手段。
목적：명학표피형태발생소（EPM）대간세포암SK-HEP-1세포생물학행위적영향。방법：구건고표체EPM적SK-HEP-1세포，real-time PCR화Western인적검측EPM재종류세포내적표체，CCK8분석화극륭형성실험검측세포적증식능력，Matrigel-transwell실험검측세포적침윤능력。결과：EPM재종류세포내적고표체불영향세포적증식능력，단명현증강종류세포적침윤능력。결론：간암종류미배경유가능통과EPM영향종류세포적생물학활성，대기작용궤제적진일보명학，장유조우천명간암발생발전적병리궤제，발현신적악성종류진단화치료수단。
Objective: To study whether epimorphin(EPM) affect SK-HEP-1 cell biology. Methods: SK-HEP-1 cells with ectopic EPM expression were constructed and identified by real-time PCR and Western blot. CCK8 anal-ysis and colony formation assay were used to detect cancer cell proliferation. The invasion activity of cancer cells were detected by Matrigel-transwell assay. Results: We first confirmed with that EPM was unregulated in hepato-cellular carcinoma(HCC) tumor stroma tissues. And, the further results suggested that ectopic expression of EPM in SK-HEP-1 cell had no effects on cancer cell proliferation, but enhanced the cell invasion activity significantly. We also showed that EPM could enhance FAK activation significantly. Conclusion: Taken together, our results re-veal that EPM was involved in HCC progression and may be a potential therapeutic target for HCC.
