CAJ | 학술논문

目的：脂肽类化合物具有抗辐射活性，通过研究脂肽类辐射防护剂H6101给药后小鼠骨髓细胞基因表达谱的变化，揭示其可能的辐射防护机制。方法：6只ICR雄性小鼠随机分为PBS对照组和H6101给药组，每组3只，给药后1 h分离骨髓细胞提取总RNA，经反转录和荧光标记后与小鼠基因表达谱芯片杂交，杂交信号经扫描仪捕获后用Genenomestudio软件进行统计分析。结果：在测定的26766个基因中，给药组与对照组之间的2倍差异表达基因为1738个，其中1041个基因表达上调，697个基因表达下调；TLR信号通路相关基因，炎性细胞因子、造血因子和细胞凋亡相关基因等的转录水平发生明显变化。结论：用基因表达谱芯片筛选出许多不同种类的与H6101辐射防护作用有关的重要基因，这为揭示脂肽分子辐射防护机制提供了研究方向。
목적：지태류화합물구유항복사활성，통과연구지태류복사방호제H6101급약후소서골수세포기인표체보적변화，게시기가능적복사방호궤제。방법：6지ICR웅성소서수궤분위PBS대조조화H6101급약조，매조3지，급약후1 h분리골수세포제취총RNA，경반전록화형광표기후여소서기인표체보심편잡교，잡교신호경소묘의포획후용Genenomestudio연건진행통계분석。결과：재측정적26766개기인중，급약조여대조조지간적2배차이표체기인위1738개，기중1041개기인표체상조，697개기인표체하조；TLR신호통로상관기인，염성세포인자、조혈인자화세포조망상관기인등적전록수평발생명현변화。결론：용기인표체보심편사선출허다불동충류적여H6101복사방호작용유관적중요기인，저위게시지태분자복사방호궤제제공료연구방향。
Objective: The lipopeptide have the activity of radioprotection. To reveal the radioprotective mecha-nism of lipopeptide H6101 in mouse, a cDNA microarrary assay was carried out to detect the effects on gene ex-pression in mouse bone marrow cells(BMC) after H6101 administration. Methods: 6 mice were randomly divided into phosphate buffer saline control group and H6101 administration group, 1 hour later, the bone marrow cell were collected respectively, total RNA was extracted and then reverse transcribed. The probes were prepared by la-beling, the arrays were hybridized against the cDNA probe mixture and the fluorescent signals were scanned. Mi-croarray analysis was performed by using mouse WG-6 v2.0 Expression BeadChip. The obtained data were ana-lyzed with Genenomestudio software. Results: Among the 26 766 tested genes, 1738 genes were found to be differ-ent by more than 2-fold between the groups. Moreover, 1041 genes were up-regulated and 697 genes were down-regulated. These genes including TLR signal pathway associated genes, inflammatory and apoptosis associated proteins. Conclusion: Profile gene chip can be used to screen out many different kinds of genes that may partici-pate in the radioprotection of H6101. The new technology may play an important role in the discovery of the gene level mechanism of lipopeptide in the future.