水产学杂志
水產學雜誌
수산학잡지
CHINESE JOURNAL OF FISHERIES
2013年
6期
40-43
,共4页
中草药%鲁氏耶尔森菌%最小抑菌浓度
中草藥%魯氏耶爾森菌%最小抑菌濃度
중초약%로씨야이삼균%최소억균농도
Chinese herbal medicine%Yersinia ruckeri%minimal inhibitory concentration
采用试管二倍稀释法测定了16种中草药单方及5种复方制剂对鲁氏耶尔森菌（Yersinia ruckeri）的最小抑菌浓度（MIC）。结果表明，黄芩（Radix Scutellariae）和诃子（Fructus Chebulae）对鲁氏耶尔森菌的抑菌作用最强，MIC均为0.028g·mL-1，可作为防治水产养殖动物耶尔森菌病害的主要备选中草药；大黄（Radix et Rhizoma Rhei）的抑菌作用较强，其MIC值为0.227g·mL-1，也可作为候选药物；青蒿（Herba Artemisiae）、金樱子（Fructus Rosae Laevigatae）、连翘（Fructus Forsythiae）、茵陈（Herba Artemisiae Scopariae）、五倍子（Galla Chinensis）及金银花（Flos Lonicerae）的抑菌作用较弱，其MIC值均为0.455g·mL-1。5种复方制剂中，大黄、黄柏（Cortex Phel-lodendri）和黄芩3种中草药以5：3：2的比例配伍对鲁氏耶尔森菌具有最佳抑菌作用，其MIC值为0.057g·mL-1，抑菌效果弱于黄芩，但远强于大黄单独使用，可为该细菌性疾病的防治提供复方配伍参考。
採用試管二倍稀釋法測定瞭16種中草藥單方及5種複方製劑對魯氏耶爾森菌（Yersinia ruckeri）的最小抑菌濃度（MIC）。結果錶明，黃芩（Radix Scutellariae）和訶子（Fructus Chebulae）對魯氏耶爾森菌的抑菌作用最彊，MIC均為0.028g·mL-1，可作為防治水產養殖動物耶爾森菌病害的主要備選中草藥；大黃（Radix et Rhizoma Rhei）的抑菌作用較彊，其MIC值為0.227g·mL-1，也可作為候選藥物；青蒿（Herba Artemisiae）、金櫻子（Fructus Rosae Laevigatae）、連翹（Fructus Forsythiae）、茵陳（Herba Artemisiae Scopariae）、五倍子（Galla Chinensis）及金銀花（Flos Lonicerae）的抑菌作用較弱，其MIC值均為0.455g·mL-1。5種複方製劑中，大黃、黃柏（Cortex Phel-lodendri）和黃芩3種中草藥以5：3：2的比例配伍對魯氏耶爾森菌具有最佳抑菌作用，其MIC值為0.057g·mL-1，抑菌效果弱于黃芩，但遠彊于大黃單獨使用，可為該細菌性疾病的防治提供複方配伍參攷。
채용시관이배희석법측정료16충중초약단방급5충복방제제대로씨야이삼균（Yersinia ruckeri）적최소억균농도（MIC）。결과표명，황금（Radix Scutellariae）화가자（Fructus Chebulae）대로씨야이삼균적억균작용최강，MIC균위0.028g·mL-1，가작위방치수산양식동물야이삼균병해적주요비선중초약；대황（Radix et Rhizoma Rhei）적억균작용교강，기MIC치위0.227g·mL-1，야가작위후선약물；청호（Herba Artemisiae）、금앵자（Fructus Rosae Laevigatae）、련교（Fructus Forsythiae）、인진（Herba Artemisiae Scopariae）、오배자（Galla Chinensis）급금은화（Flos Lonicerae）적억균작용교약，기MIC치균위0.455g·mL-1。5충복방제제중，대황、황백（Cortex Phel-lodendri）화황금3충중초약이5：3：2적비례배오대로씨야이삼균구유최가억균작용，기MIC치위0.057g·mL-1，억균효과약우황금，단원강우대황단독사용，가위해세균성질병적방치제공복방배오삼고。
The minimal inhibitory concentration of 16 kinds of Chinese herbal medicines and 5 kinds of Chinese herbal mixtures on bacterium Yersinia ruckeri was determined in vitro by a microdilution method. The results showed that Radix Scutellariae and Fructus Chebulae had the strongest inhibitory effect on Yersinia ruckeri with the MIC of 0.028g·mL-1, indicating that they were the better herbal drugs that cure the disease caused by the Yersinia ruckeri in aquatic animals. Radix et Rhizoma Rhei had stronger inhibitory ef-fect on Y. ruckeri with the MIC as 0.227g·mL-1 and was used as one of the compound herbal medicine to treat this bacterial disease. Herba Artemisiae, Fructus Rosae Laevigatae, Fructus Forsythiae, Herba Artemisiae Scopariae, Galla Chinensis, and Flos Lonicerae had weak inhibitory effect on Yersinia ruckeri, with the MIC as 0.455g·mL-1. The mixture of Radix et Rhizoma Rhei, Cortex Phellodendri and Radix Scutellariae with the proportion of 5:3:2 was shown to have the best MIC 0.057 g·mL-1on Yersinia ruckeri. Though less than Radix Scutellariae, this mixture had stronger than the Radix et Rhizoma Rhei only, indicating that the compound can be used to pre-vent this kind of fish diseases.
