CAJ | 학술논문

在无菌条件下研究了伯克氏菌对镉胁迫下水稻种子萌发及初生幼苗耐镉性的影响。结果表明：伯克氏菌D54能在含500 mg·L-1Cd的培养基中正常生长，显示出极强的耐镉能力。在50 mg·L-1的镉胁迫下，接种伯克氏菌能显著提高水稻种子的萌发率，但在其他镉处理下，接种伯克氏菌对种子萌发无显著影响。接种伯克氏菌对水稻种子的发芽指数没有影响，但在10 mg·L-1Cd处理下，接种伯克氏菌能显著提高水稻种子的活力指数和初生幼苗的根长、根表面积、根尖总数和耐性系数。接种伯克氏菌对初生幼苗的芽鲜重无影响，但能显著提高根鲜重。在50 mg·L-1和100 mg·L-1的高浓度镉处理下，接种伯克氏菌对根系生长没有影响。
재무균조건하연구료백극씨균대력협박하수도충자맹발급초생유묘내력성적영향。결과표명：백극씨균D54능재함500 mg·L-1Cd적배양기중정상생장，현시출겁강적내력능력。재50 mg·L-1적력협박하，접충백극씨균능현저제고수도충자적맹발솔，단재기타력처리하，접충백극씨균대충자맹발무현저영향。접충백극씨균대수도충자적발아지수몰유영향，단재10 mg·L-1Cd처리하，접충백극씨균능현저제고수도충자적활력지수화초생유묘적근장、근표면적、근첨총수화내성계수。접충백극씨균대초생유묘적아선중무영향，단능현저제고근선중。재50 mg·L-1화100 mg·L-1적고농도력처리하，접충백극씨균대근계생장몰유영향。
We studied the effect of Burkholderia sp.D54 on rice seed germination and Cd-tolerance of rice seedlings under gnotobiotic Cd stress. The results showed that Burkholderia sp.D54 could grow normally under 500 mg·L-1 Cd stress. Inoculation of Burkholderia sp.D54 sig-nificantly increased rice seed germination rate under 50 mg·L-1Cd stress,but it had no effect on germination index of any Cd treatment. Inoc-ulation with Burkholderia sp.D54 could significantly improve vigour index of rice seeds and total root length, surface area, tip numbers and tolerance index of rice seedlings under 10 mg·L-1Cd stress. Inoculation of Burkholderia sp.D54 could promote the growth of rice roots, but had no effect on the shoot. There were no significant difference of root length, surface area, tip numbers between inoculated and uninoculated one under 50 mg·L-1and 100 mg·L-1 Cd stress.