昆明理工大学学报(自然科学版)
昆明理工大學學報(自然科學版)
곤명리공대학학보(자연과학판)
JOURNAL OF KUNMING UNIVERSITY OF SCIENCE AND TECHNOLOGY(SCIENCE AND TECHNOLOGY)
2013年
6期
96-101
,共6页
许志刚%庞宏斌%刘智敏%陈云丽
許誌剛%龐宏斌%劉智敏%陳雲麗
허지강%방굉빈%류지민%진운려
普洱茶%儿茶素%萃取%高效液相色谱法
普洱茶%兒茶素%萃取%高效液相色譜法
보이다%인다소%췌취%고효액상색보법
Pu’er tea%catechin%extraction%high performance liquid chromatography
分别采用微波、超声和溶剂回流三种方法对普洱茶样品中的三种儿茶素进行提取,并结合高效液相色谱进行定量分析,结果表明:微波提取法高效、快捷,表没食子儿茶素(E GC )、表儿茶素(EC)和表没食子儿茶素没食子酸酯(EGCG)三种儿茶素的含量分别为2.13%、2.94%和7.26%.扫描电镜图进一步证实:微波提取使茶叶样品表观结构明显侵蚀,并呈现出花纹状,这有利于分析物的提取.
分彆採用微波、超聲和溶劑迴流三種方法對普洱茶樣品中的三種兒茶素進行提取,併結閤高效液相色譜進行定量分析,結果錶明:微波提取法高效、快捷,錶沒食子兒茶素(E GC )、錶兒茶素(EC)和錶沒食子兒茶素沒食子痠酯(EGCG)三種兒茶素的含量分彆為2.13%、2.94%和7.26%.掃描電鏡圖進一步證實:微波提取使茶葉樣品錶觀結構明顯侵蝕,併呈現齣花紋狀,這有利于分析物的提取.
분별채용미파、초성화용제회류삼충방법대보이다양품중적삼충인다소진행제취,병결합고효액상색보진행정량분석,결과표명:미파제취법고효、쾌첩,표몰식자인다소(E GC )、표인다소(EC)화표몰식자인다소몰식자산지(EGCG)삼충인다소적함량분별위2.13%、2.94%화7.26%.소묘전경도진일보증실:미파제취사다협양품표관결구명현침식,병정현출화문상,저유리우분석물적제취.
The extraction of three catechins of epigallocatechin (EGC),epicatechin (EC)and epigallocatechin gallate (EGCG)in Pu’er tea is investigated by the methods of microwave assisted extraction,ultrasonic extrac-tion and solvent reflux extraction,respectively.The three catechins are then determined by high performance liq-uid chromatography.The results indicate that microwave assisted extraction method is faster and more efficient. The contents of EGC,EC and EGCG are 2.1 3%,2.94% and 7.26%,respectively.It is thereafter confirmed by the scanning electron micrographs that the tea sample surface structure is apparently corroded after microwave assisted extraction.The visible pattern structure is conducive to the extraction of analytes.