CAJ | 학술논문

研究乳铁蛋白（lactoferrin，LF）对脂多糖诱导的急性肺损伤（acute lung injury，ALI）的保护作用，并探讨其可能机制．清洁级雄性昆明小鼠腹腔注射LPS 5 mg/kg前1 h或注射LPS后1 h给予LF 5 mg/body，同时以2 mg/kg地塞米松治疗组为阳性对照组．给予LPS 12 h后，收集左肺肺泡灌洗液．检测白细胞介素-10及肿瘤坏死因子-α的含量、总蛋白含量；离心后将沉淀重悬检测灌洗液中总细胞数．取右肺上叶速冻于-80℃冰箱，检测肺组织中髓过氧化物酶含量；中叶称湿、干重，计算肺湿/干重比；下叶进行HE 染色，观察肺组织病理学改变．结果发现，LF能明显减轻肺泡腔出血、水肿、炎细胞浸润等病理过程，肺组织病理学评分明显降低（P＜0.05）；LF能降低肺组织MPO活性、肺泡灌洗液中总细胞、总蛋白含量、W/D和TNF-α含量（P＜0.05）；增加肺组织IL-10含量（P＜0.05）．这些结果说明，LF对LPS诱导的急性肺损伤有显著的预防和治疗作用．
연구유철단백（lactoferrin，LF）대지다당유도적급성폐손상（acute lung injury，ALI）적보호작용，병탐토기가능궤제．청길급웅성곤명소서복강주사LPS 5 mg/kg전1 h혹주사LPS후1 h급여LF 5 mg/body，동시이2 mg/kg지새미송치료조위양성대조조．급여LPS 12 h후，수집좌폐폐포관세액．검측백세포개소-10급종류배사인자-α적함량、총단백함량；리심후장침정중현검측관세액중총세포수．취우폐상협속동우-80℃빙상，검측폐조직중수과양화물매함량；중협칭습、간중，계산폐습/간중비；하협진행HE 염색，관찰폐조직병이학개변．결과발현，LF능명현감경폐포강출혈、수종、염세포침윤등병리과정，폐조직병이학평분명현강저（P＜0.05）；LF능강저폐조직MPO활성、폐포관세액중총세포、총단백함량、W/D화TNF-α함량（P＜0.05）；증가폐조직IL-10함량（P＜0.05）．저사결과설명，LF대LPS유도적급성폐손상유현저적예방화치료작용．
This study aims to investigate the protective effect and mechanisms of lactoferrin (LF)on acute lung injury (ALI)induced by lipopolysaccharide (LPS).Kunming male mice of clean grade are given LF (5 mg/body)by intraperitoneal injection of 5 mg/kg LPS at 1 h before or after LPS administration.In the meanwhile, the positive control group is injected 2 mg/kg.1 2 hours after LPS administration,the bronchoalveolar lavage flu-id of the left lung are collected to test the content of TNF-α,IL-1 0,the concentration of protein and the sedi-mentary cells.The right lung is harvested for histology and determination of the myeloperoxidase activity and W/D.The results show that LF significantly attenuates the scores of the lung tissues,myeloperoxidase activity,the total cells,the total protein concentration,W/D and the content of TNF-α(P<0.05 ),while increasing the content of IL-1 0 (P<0.05 ).These results suggest that LF could inhibit the inflammatory reaction of the mice with LPS-induced ALI.