郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
1期
15-17
,共3页
郭新宾%邓鑫%张建宁%宋来君
郭新賓%鄧鑫%張建寧%宋來君
곽신빈%산흠%장건저%송래군
内皮祖细胞%CD34 +细胞%脑创伤%大鼠
內皮祖細胞%CD34 +細胞%腦創傷%大鼠
내피조세포%CD34 +세포%뇌창상%대서
endothelial progenitor cell%CD34 +cell%brain injury%rat
目的：研究大鼠脑创伤后外周血及创伤区脑组织中CD34＋细胞数量变化的规律和意义。方法：取雄性Wistar大鼠98只，随机分为创伤组和假手术组，创伤组大鼠制备液压脑创伤模型。每组各抽取7只大鼠，分别于创伤前和创伤后第1、2、3、5和7天取内眦静脉血进行CD34＋细胞计数。伤后第0、1、3、7、14和21天，每组各随机抽取7只大鼠处死，在脑损伤节段取材，采用免疫组化SP染色法检测CD34。结果：与假手术组比较，伤后第1天创伤组大鼠外周血CD34＋细胞计数即显著增加，伤后第2天达到高峰，然后逐渐恢复至正常水平（ F组间＝14．695， F时间＝27．307，F交互＝4．779，P＜0．001）；伤后第1天，创伤区脑组织CD34＋细胞增多，伤后第3天显著增加，伤后第7天达到峰值，随后有所下降，但仍维持在较高水平（F组间＝561．542， F时间＝62．374，F交互＝58．222，P＜0．001）。结论：脑创伤后外周血CD34＋细胞明显动员，并可能归巢到创伤区域，参与血管新生。
目的：研究大鼠腦創傷後外週血及創傷區腦組織中CD34＋細胞數量變化的規律和意義。方法：取雄性Wistar大鼠98隻，隨機分為創傷組和假手術組，創傷組大鼠製備液壓腦創傷模型。每組各抽取7隻大鼠，分彆于創傷前和創傷後第1、2、3、5和7天取內眥靜脈血進行CD34＋細胞計數。傷後第0、1、3、7、14和21天，每組各隨機抽取7隻大鼠處死，在腦損傷節段取材，採用免疫組化SP染色法檢測CD34。結果：與假手術組比較，傷後第1天創傷組大鼠外週血CD34＋細胞計數即顯著增加，傷後第2天達到高峰，然後逐漸恢複至正常水平（ F組間＝14．695， F時間＝27．307，F交互＝4．779，P＜0．001）；傷後第1天，創傷區腦組織CD34＋細胞增多，傷後第3天顯著增加，傷後第7天達到峰值，隨後有所下降，但仍維持在較高水平（F組間＝561．542， F時間＝62．374，F交互＝58．222，P＜0．001）。結論：腦創傷後外週血CD34＋細胞明顯動員，併可能歸巢到創傷區域，參與血管新生。
목적：연구대서뇌창상후외주혈급창상구뇌조직중CD34＋세포수량변화적규률화의의。방법：취웅성Wistar대서98지，수궤분위창상조화가수술조，창상조대서제비액압뇌창상모형。매조각추취7지대서，분별우창상전화창상후제1、2、3、5화7천취내자정맥혈진행CD34＋세포계수。상후제0、1、3、7、14화21천，매조각수궤추취7지대서처사，재뇌손상절단취재，채용면역조화SP염색법검측CD34。결과：여가수술조비교，상후제1천창상조대서외주혈CD34＋세포계수즉현저증가，상후제2천체도고봉，연후축점회복지정상수평（ F조간＝14．695， F시간＝27．307，F교호＝4．779，P＜0．001）；상후제1천，창상구뇌조직CD34＋세포증다，상후제3천현저증가，상후제7천체도봉치，수후유소하강，단잉유지재교고수평（F조간＝561．542， F시간＝62．374，F교호＝58．222，P＜0．001）。결론：뇌창상후외주혈CD34＋세포명현동원，병가능귀소도창상구역，삼여혈관신생。
Aim:To detect the changes of CD 34 +cells in peripheral blood and in posttraumatic brain tissue of rats with traumatic brain injury(TBI).Methods:A total of 98 rats were randomly assigned to control and TBI groups ,49 rats in each group .Fluid percussion injury was performed over the right parietal lobe in TBI group .Seven in each group were sampled randomly to collect blood samples from retro-orbital venous plexus before TBI , and at the 1st,2nd,3rd, 5th and 7th day after TBI, and CD34 +cells in peripheral blood were evaluated by flow cytometry .At the 1st, 3rd, 7th, 14th, and 21st day after TBI, 7 rats from the 2 groups were sampled respectively and sacrificed to detect CD 34 +cells in brain tissue using immunohistochemistry staining .Results:Compared with those of the control group , the number of CD34 +cells in peripheral blood began to increase significantly in TBI group at the 1st day after TBI,reached the peak at the 2nd day after TBI,then decreased to the normal level (Fgroup =14.695, Ftime =27.307,Finteraction =4.779,P<0.001);the number of CD34 +cells in the injured brain tissue began to increase at the 1st day after TBI,and reached the peak at the 7th day after TBI,then decreased gradually , but stabilised at a higher level ( Fgroup =561.542, Ftime =62.374, Finteraction =58.222, P<0.001).Conclusion:CD34 +cells in peripheral blood are mobilized after TBI ,and maybe home to the injured tissue and play an important role in angiogenesis .
