CAJ | 학술논문

为进一步探讨软枝黄蝉杀虫活性成分，以菜青虫为靶标昆虫，采用生物活性示踪法分离鉴定软枝黄蝉杀虫活性成分，采用小叶碟添加法测定活性成分对菜青虫幼虫的杀虫活性；3种活性成分鉴定分别为黄蝉花定、黄蝉花辛和黄蝉花素。活性测试结果表明，3种活性成分对菜青虫5龄幼虫均有很强的拒食作用，处理后48hAFC50值分别为0.071，0.045和0.103mg/mL，同时，3种化合物对菜青虫还有较强的毒杀作用，其中，以黄蝉花素毒杀作用最强，其LC50值仅为0.026mg/mL，优于对照药剂川楝素(0.033mg/mL)；环烯醚萜类化合物可能是软枝黄蝉主要的杀虫活性成分。
위진일보탐토연지황선살충활성성분，이채청충위파표곤충，채용생물활성시종법분리감정연지황선살충활성성분，채용소협설첨가법측정활성성분대채청충유충적살충활성；3충활성성분감정분별위황선화정、황선화신화황선화소。활성측시결과표명，3충활성성분대채청충5령유충균유흔강적거식작용，처리후48hAFC50치분별위0.071，0.045화0.103mg/mL，동시，3충화합물대채청충환유교강적독살작용，기중，이황선화소독살작용최강，기LC50치부위0.026mg/mL，우우대조약제천련소(0.033mg/mL)；배희미첩류화합물가능시연지황선주요적살충활성성분。
In order to clarify the insecticidal activities and active compounds of A llamanda c athartic a Linn, three insecticidal ingredients were isolated from ethanol extract by bioassay-guided fractionation. The antifeeding activities and insecticidal activities of three compounds against Pieris rapae larvae were tested by leaf disk method. Their chemical structures were identified to be allamandin, allamandicin and allamdin repectively by MS, 1H NMR. The AFC50 values of antifeedant of 3 insecticidal compounds against fifth instar larvae of P. rapae were 0.071, 0.045 and 0.103 mg/mL after 48 hours respectively. allamdin showed insecticidal activity against fifth instar larvae of P. rapae and its LC50 value was 0.026 mg/mL, which was superior to positive control toosendanin (0.033 mg/mL). Iridoids may be the main insecticidal ingredients of A . c athartic a Linn.