CAJ | 학술논문

以巴西橡胶树花芽为材料，利用RT-PCR技术从总RNA中扩增获得1个MA DS基因。 cDNA长度1063 bp，开放阅读框717 bp，编码239个氨基酸，命名为HbMADS-27。在HbMADS-27氨基酸序列中具有1个较强的跨膜区，二级结构分析结果表明其属于混合型蛋白。 HbMADS-27在N端前34～50氨基酸区域内为较强的疏水区，但整条多肽链表现为亲水性。对20个MADS蛋白进行系统进化分析，结果发现，巴西橡胶树的HbMADS-27基因与蓖麻的MA DS-27基因亲缘关系最近，而与拟南芥MA DS-27基因亲缘关系相对较远。
이파서상효수화아위재료，이용RT-PCR기술종총RNA중확증획득1개MA DS기인。 cDNA장도1063 bp，개방열독광717 bp，편마239개안기산，명명위HbMADS-27。재HbMADS-27안기산서렬중구유1개교강적과막구，이급결구분석결과표명기속우혼합형단백。 HbMADS-27재N단전34～50안기산구역내위교강적소수구，단정조다태련표현위친수성。대20개MADS단백진행계통진화분석，결과발현，파서상효수적HbMADS-27기인여비마적MA DS-27기인친연관계최근，이여의남개MA DS-27기인친연관계상대교원。
A novel full-length MADS gene named HbMADS-27 was obtained by using reverse transcription-polymerase chain reaction (RT-PCR) from total RNA of flower bud of rubber tree (Hevea brasiliensis). HbMADS-27 was 1 063 bp long containing a 717 bp open reading frame (ORF). It encoded 239 amino acids. One strong transmembrane structure was predicted within HbMADS-27. The result of secondary structure predicted showed that the protein was classified as mixed type. There was a strong hydrophobic region in the N-terminal 34~50 amino acids of HbMA DS-27, but the entire polypeptide chain was hydrophilic. The result of phylogenetic tree indicated that HbMA DS-27 displayed a high degree of sequence similarity to RcMADS-27, and had a low degree of sequence similarity to AtMADS-27.